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リコンビナントRabMAb

Anti-SLUG 抗体 [EPR25113-46] - BSA and Azide free (ab302781)

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Western blot - Anti-SLUG antibody [EPR25113-46] (BSA and Azide free) (AB302781)
  • Western blot - Anti-SLUG antibody [EPR25113-46] (BSA and Azide free) (AB302781)
  • Anti-SLUG antibody [EPR25113-46] (BSA and Azide free) (AB302781)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25113-46] to SLUG - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-SLUG antibody [EPR25113-46] - BSA and Azide free
    SLUG 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25113-46] to SLUG - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt (Intra),IHC-P or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: A-204 whole cell lysate. His-tagged human SLUG recombinant protein.

  • 特記事項

    AB302781 is a carrier free version of AB302780. 

    Ab302780 does not react in Western blot in mouse and rat samples. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302781の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
追加情報
Is unsuitable for Flow Cyt (Intra),IHC-P or IP.

ターゲット情報

  • 機能

    Transcriptional repressor. Involved in the generation and migration of neural crest cells.

  • 組織特異性

    Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.

  • 関連疾患

    Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.

  • 配列類似性

    Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • MGC10182 antibody
      • Neural crest transcription factor Slug antibody
      • Protein snail homolog 2 antibody
      • Slug (chicken homolog) zinc finger protein antibody
      • Slug homolog zinc finger protein antibody
      • Slug zinc finger protein antibody
      • SLUGH antibody
      • SLUGH 1 antibody
      • SLUGH1 antibody
      • SNAI 2 antibody
      • Snai2 antibody
      • SNAI2_HUMAN antibody
      • Snail 2 antibody
      • Snail homolog 2 antibody
      • Snail2 antibody
      • WS 2D antibody
      • WS2D antibody
      • Zinc finger protein SLUG antibody
      • Zinc finger protein SNAI2 antibody
      see all

    画像

    • Western blot - Anti-SLUG antibody [EPR25113-46] (BSA and Azide free) (AB302781)
      Western blot - Anti-SLUG antibody [EPR25113-46] (BSA and Azide free) (AB302781)
      All lanes : Anti-SLUG antibody [EPR25113-46] (ab302780) at 1/1000 dilution

      Lane 1 : A-204 (Human muscle rhabdomyosarcoma), whole cell lysate
      Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 29 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?


      Exposure time: 48 seconds


      This data was developed using ab302780, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST. 

      Negative control: MCF7 (PMID:19756381).

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Western blot - Anti-SLUG antibody [EPR25113-46] (BSA and Azide free) (AB302781)
      Western blot - Anti-SLUG antibody [EPR25113-46] (BSA and Azide free) (AB302781)
      All lanes : Anti-SLUG antibody [EPR25113-46] (ab302780) at 1/1000 dilution

      Lane 1 : His-tagged human SLUG recombinant protein, 10 ng
      Lane 2 : His-tagged human SNAI1 recombinant protein, 10 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 29 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab302780, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST. 

      This antibody does not cross-react with human SNAI1.

      Both recombinant proteins were made in-house and expressed from the E. coli expression systems.

    • Anti-SLUG antibody [EPR25113-46] (BSA and Azide free) (AB302781)
      Anti-SLUG antibody [EPR25113-46] (BSA and Azide free) (AB302781)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302781 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302781 は論文での使用が確認できていません。

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