Anti-SLUG 抗体 [EPR25113-46] (ab302780)
![Western blot - Anti-SLUG antibody [EPR25113-46] (ab302780)](https://www.abcam.co.jp/ps/products/302/ab302780/Images/AB302780-490604-anti-slug-antibody-epr25113-46-western-blot-a204.jpg "Western blot - Anti-SLUG antibody [EPR25113-46] (ab302780)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25113-46] to SLUG
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-SLUG antibody [EPR25113-46]
SLUG 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25113-46] to SLUG -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt (Intra),IHC-P or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- WB: A-204 whole cell lysate. His-tagged human SLUG recombinant protein.
-
特記事項
AB302780 does not react in Western blot in mouse and rat samples.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25113-46 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302780の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
|
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa). |
ターゲット情報
-
機能
Transcriptional repressor. Involved in the generation and migration of neural crest cells. -
組織特異性
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
関連疾患
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. -
配列類似性
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 6591 Human
- Omim: 602150 Human
- SwissProt: O43623 Human
- Unigene: 360174 Human
-
別名
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
see all
画像
-
Western blot - Anti-SLUG antibody [EPR25113-46] (ab302780)All lanes : Anti-SLUG antibody [EPR25113-46] (ab302780) at 1/1000 dilution
Lane 1 : A-204 (Human muscle rhabdomyosarcoma), whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking and dilution buffer: 5% NFDM/TBST.
Negative control: MCF7 (PMID:19756381).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
-
![Western blot - Anti-SLUG antibody [EPR25113-46] (AB302780)](https://www.abcam.co.jp/ps/products/302/ab302780/Images/AB302780-490605-anti-slug-antibody-epr25113-46-western-blot-human-slug.jpg)
Western blot - Anti-SLUG antibody [EPR25113-46] (AB302780)All lanes : Anti-SLUG antibody [EPR25113-46] (ab302780) at 1/1000 dilution
Lane 1 : His-tagged human SLUG recombinant protein, 10 ng
Lane 2 : His-tagged human SNAI1 recombinant protein, 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
This antibody does not cross-react with human SNAI1.
Both recombinant proteins were made in-house and expressed from the E. coli expression systems.
-
![Anti-SLUG antibody [EPR25113-46] (AB302780)](https://www.abcam.co.jp/ps/products/302/ab302780/Images/AB302780-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-SLUG antibody [EPR25113-46] (AB302780)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (3)
ab302780 は 3 報の論文で使用されています。
- Shi KH et al. KRT80 expression works as a biomarker and a target for differentiation in gastric cancer. Histol Histopathol 39:117-130 (2024). PubMed: 37129345
- Wang LL et al. The promoting effects of Grin2d expression in tumorigenesis and the aggressiveness of esophageal cancer. Histol Histopathol 39:659-670 (2024). PubMed: 37982578
- Xu X et al. TPPP3 promote epithelial-mesenchymal transition via Snail1 in glioblastoma. Sci Rep 13:17960 (2023). PubMed: 37863960