Anti-SLUG 抗体 (ab27568)
Key features and details
- Rabbit polyclonal to SLUG
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SLUG antibody
SLUG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SLUG -
由来種
Rabbit -
特異性
ab27568 detects the overexpressing human Slug protein, however we have no data on the detection of the endogenous Human protein with this current batch. ab27568 does not cross react with overexpressed SNAIL protein in cell lysates. -
アプリケーション
適用あり: WB, IHC-Frmore details
適用なし: ICC -
種交差性
交差種: Mouse, Rat, Chicken, Human
交差が予測される動物種: Cow, Dog, Pig, Xenopus laevis
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免疫原
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27568の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa).
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| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| 特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa). |
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IHC-Fr
Use at an assay dependent concentration. |
追加情報
Is unsuitable for ICC.
ターゲット情報
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機能
Transcriptional repressor. Involved in the generation and migration of neural crest cells. -
組織特異性
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
関連疾患
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. -
配列類似性
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 432368 Chicken
- Entrez Gene: 6591 Human
- Entrez Gene: 20583 Mouse
- Entrez Gene: 25554 Rat
- Entrez Gene: 379440 Xenopus laevis
- Omim: 602150 Human
- SwissProt: Q3MHQ4 Cow
- SwissProt: O43623 Human
see all -
別名
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-SLUG antibody (ab27568)This image is courtesy of Don Newgreen and Dong Cheng Zhang, Murdoch Childrens Research InstituteTransverse section showing Slug-expressing neural crest cells (green) in the dorsal neural tube (NT) in the hindbrain of a quail embryo. 4% PFA fixed embryonic day 1.7 Japanese Quail embryo, frozen in Tissutek, sectioned 12 um. Ab27568 1/400, incubated overnight. Goat anti-rabbit: Alexa488 (Molecular Probes) 1/1000 incubated for 2 hours.
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Western blot - Anti-SLUG antibody (ab27568)All lanes : Anti-SLUG antibody (ab27568) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaSecondary antibody - goat anti-rabbit HRP (preadsorbed)
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Western blot - Anti-SLUG antibody (ab27568)This image is courtesy of Archana Dhasarathy, Ph.D., NCAll lanes : Anti-SLUG antibody (ab27568) at 1/1000 dilution
Lane 1 : MCF7 cell lysate overexpressing SLUG protein
Lane 2 : MCF7 lysate overexpressing SNAIL protein
Lane 3 : MCF7 lysate tranfected with vector control plasmid
Secondary
All lanes : HRP-conjugated anti-rabbit antibody at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
ab27568 detects a band of 34 kDa in MCF7 cells that have been transfected with a vector that overexpresses the SLUG protein. This antibody does not crossreact with the SNAIL protein, as it did not recognize this protein in MCF7 cells overexpressing SNAIL. ab27568 was unable to detect endogenous SLUG in MDA-MB231 cells (not shown).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (142)
ab27568 は 142 報の論文で使用されています。
- Lin Q et al. Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling. Exp Ther Med 23:3 (2022). PubMed: 34815755
- Huang CS et al. Long Noncoding RNA LINC02470 Sponges MicroRNA-143-3p and Enhances SMAD3-Mediated Epithelial-to-Mesenchymal Transition to Promote the Aggressive Properties of Bladder Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35205713
- Yu Y et al. Synergistic effect of Tripterygium glycosides and cisplatin on drug-resistant human epithelial ovarian cancer via ILK/GSK3β/Slug signal pathway. Am J Transl Res 14:2051-2062 (2022). PubMed: 35422913
- Wang LN et al. TGF-β1/SH2B3 axis regulates anoikis resistance and EMT of lung cancer cells by modulating JAK2/STAT3 and SHP2/Grb2 signaling pathways. Cell Death Dis 13:472 (2022). PubMed: 35589677
- Nam HJ et al. GPR110 promotes progression and metastasis of triple-negative breast cancer. Cell Death Discov 8:271 (2022). PubMed: 35614051