Anti-SLUG 抗体 (ab106077)
Key features and details
- Rabbit polyclonal to SLUG
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SLUG antibody
SLUG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SLUG -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chimpanzee, Macaque monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human SLUG aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab133782) -
特記事項
This antibody has only been tested on overexpressing cell lysates; we have no data on the detection of the endogenous protein.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106077の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa). |
ターゲット情報
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機能
Transcriptional repressor. Involved in the generation and migration of neural crest cells. -
組織特異性
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
関連疾患
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. -
配列類似性
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6591 Human
- Entrez Gene: 20583 Mouse
- Omim: 602150 Human
- SwissProt: O43623 Human
- SwissProt: P97469 Mouse
- Unigene: 360174 Human
- Unigene: 4272 Mouse
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別名
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
see all
画像
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Western blot - Anti-SLUG antibody (ab106077)All lanes : Anti-SLUG antibody (ab106077) at 1 µg/ml
Lane 1 : MCF7 cell lysate overexpressing SLUG protein
Lane 2 : MCF7 cell lysate overexpressing SNAIL protein
Lane 3 : MCF7 cell lysate transfected with vector control plasmid
Lane 4 : Untransfected MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes -
Western blot - Anti-SLUG antibody (ab106077)This image is courtesy of an anonymous AbreviewAll lanes : Anti-SLUG antibody (ab106077) at 1/2000 dilution
Lane 1 : MDA-MB-231 whole cell lysate
Lane 2 : MDA-MB-231 whole cell lysate - sh Slug 1
Lane 3 : MDA-MB-231 whole cell lysate - sh Slug 2
Lane 4 : MDA-MB-231 whole cell lysate - sh Snail 1
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 35,65 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific binding)
Exposure time: 30 secondsBlocked with 5% milk for 1 hour at 22°C.
Primary incubation at 4°C for 18 hours.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (20)
ab106077 は 20 報の論文で使用されています。
- Yang Y et al. Tumor Suppressor microRNA-138 Suppresses Low-Grade Glioma Development and Metastasis via Regulating IGF2BP2. Onco Targets Ther 13:2247-2260 (2020). PubMed: 32214825
- Tian S et al. SERPINH1 regulates EMT and gastric cancer metastasis via the Wnt/ß-catenin signaling pathway. Aging (Albany NY) 12:3574-3593 (2020). PubMed: 32091407
- Lv X et al. Circ-MMP2 (circ-0039411) induced by FOXM1 promotes the proliferation and migration of lung adenocarcinoma cells in vitro and in vivo. Cell Death Dis 11:426 (2020). PubMed: 32513952
- Chu G et al. Role and Mechanism of Rhizopus Nigrum Polysaccharide EPS1-1 as Pharmaceutical for Therapy of Hepatocellular Carcinoma. Front Bioeng Biotechnol 8:509 (2020). PubMed: 32582655
- Liu K et al. The Sp1/FOXC1/HOTTIP/LATS2/YAP/ß-catenin cascade promotes malignant and metastatic progression of osteosarcoma. Mol Oncol 14:2678-2695 (2020). PubMed: 32634265
