Anti-SLP76 (phospho Y113) 抗体 [EPR2851] (ab75990)
![Western blot - Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)](https://www.abcam.co.jp/ps/products/75/ab75990/Images/ab75990-91240-anti-slp76-phospho-y113-antibody-epr2851-western-blot.jpg "Western blot - Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2851] to SLP76 (phospho Y113)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-SLP76 (phospho Y113) antibody [EPR2851]
SLP76 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2851] to SLP76 (phospho Y113) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Rabbit
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- Jurkat cytoplasmic lysate treated with pervanadate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2851 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75990の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/5000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
|
| 特記事項 |
|---|
|
WB
1/5000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa). |
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.
ターゲット情報
-
機能
Involved in T-cell antigen receptor mediated signaling. -
組織特異性
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines. -
配列類似性
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH2 domain. -
ドメイン
The SH2 domain mediates interaction with SHB. -
翻訳後修飾
Phosphorylated after T-cell receptor activation by ZAP-70. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 3937 Human
- Omim: 601603 Human
- SwissProt: Q13094 Human
- Unigene: 304475 Human
-
別名
- 76 kDa tyrosine phosphoprotein antibody
- CG8697 antibody
- LCP 2 antibody
see all
画像
-
Western blot - Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)All lanes : Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990) at 1/10000 dilution
Lane 1 : Jurkat cell lysate - untreated
Lane 2 : Jurkat cell lysate - treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted? -
![Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)](https://www.abcam.co.jp/ps/products/75/ab75990/Images/ab75990-2-benefits-of-recombinant-antibodies.png)
Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab75990 は 3 報の論文で使用されています。
- Hoshino A et al. Dysregulation of Epstein-Barr Virus Infection in Hypomorphic ZAP70 Mutation. J Infect Dis 218:825-834 (2018). PubMed: 29684201
- Cimo AM et al. CD25 and CD69 induction by a4ß1 outside-in signalling requires TCR early signalling complex proteins. Biochem J 454:109-21 (2013). WB ; Human . PubMed: 23758320
- Köhler K et al. Matched sizes of activating and inhibitory receptor/ligand pairs are required for optimal signal integration by human natural killer cells. PLoS One 5:e15374 (2010). WB ; Human . PubMed: 21179506