Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-SLP76 抗体 [EPR2549(2)] (ab109254)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
  • Western blot - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
  • Anti-SLP76 antibody [EPR2549(2)] (ab109254)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2549(2)] to SLP76
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SLP76 antibody [EPR2549(2)]
    SLP76 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2549(2)] to SLP76

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • MOLT4 or HuT78 cell lysates; Human tonsil tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR2549(2)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109254の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Involved in T-cell antigen receptor mediated signaling.

  • 組織特異性

    Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.

  • 配列類似性

    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH2 domain.

  • ドメイン

    The SH2 domain mediates interaction with SHB.

  • 翻訳後修飾

    Phosphorylated after T-cell receptor activation by ZAP-70.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 76 kDa tyrosine phosphoprotein antibody
      • CG8697 antibody
      • LCP 2 antibody
      • LCP2 antibody
      • LCP2_HUMAN antibody
      • Lymphocyte cytosolic protein 2 antibody
      • SH2 domain containing leukocyte protein 76 KD antibody
      • SH2 domain containing leukocyte protein of 76kD antibody
      • SH2 domain containing leukocyte protein of 76kDa antibody
      • SH2 domain-containing leukocyte protein of 76 kDa antibody
      • SLP 76 antibody
      • SLP 76 tyrosine phosphoprotein antibody
      • SLP-76 antibody
      • SLP-76 tyrosine phosphoprotein antibody
      • SLP76 antibody
      • SLP76 tyrosine phosphoprotein antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)

      ab109254 at 1/100 dilution, staining SLP76 in Human tonsil, by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)

      ab109254 showing positive staining in Normal human spleen tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
      Western blot - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
      All lanes : Anti-SLP76 antibody [EPR2549(2)] (ab109254) at 1/1000 dilution

      Lane 1 : HuT-78 cell lysate
      Lane 2 : MOLT-4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)

      ab109254 showing positive staining in Normal human colon tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)

      ab109254 showing negative staining in Normal human brain tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] (ab109254)

      ab109254 showing negative staining in Normal human heart tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-SLP76 antibody [EPR2549(2)] (ab109254)
      Anti-SLP76 antibody [EPR2549(2)] (ab109254)

    参考文献 (1)

    ab109254 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109254 は 1 報の論文で使用されています。

    • Hoshino A  et al. Dysregulation of Epstein-Barr Virus Infection in Hypomorphic ZAP70 Mutation. J Infect Dis 218:825-834 (2018). PubMed: 29684201

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab109254.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com