Anti-SLK 抗体 [EPR25108-252] (ab302536)
![Western blot - Anti-SLK antibody [EPR25108-252] (ab302536)](https://www.abcam.co.jp/ps/products/302/ab302536/Images/AB302536-2-anti-slk-antibody-epr25108252-western-blot-hela-human-cervical-adenocarcinoma-epithelial-cell-transfected-with-scrambled-sirna-control-whole-cell-lysate.jpg "Western blot - Anti-SLK antibody [EPR25108-252] (ab302536)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25108-252] to SLK
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SLK antibody [EPR25108-252]
SLK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25108-252] to SLK -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Whole cell lysates: HeLa (human cervical adenocarcinoma epithelial cell), 293T (human embryonic kidney epithelial cell), NIH/3T3 (mouse embryonic fibroblast), PC-12 (rat adrenal gland pheochromocytoma), HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control, HeLa transfected with siRNA specifically targeting SLK.
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特記事項
Ab302536 does not react in: IHC-P for human, mouse, and rat; ICC for human, and mouse; Flow cyt. intra. for human, and mouse; IP for human, and mouse species.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25108-252 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302536の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 143 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 143 kDa). |
ターゲット情報
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関連性
SLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 9748 Human
- Entrez Gene: 20874 Mouse
- Entrez Gene: 54308 Rat
- SwissProt: Q9H2G2 Human
- SwissProt: O54988 Mouse
- SwissProt: O08815 Rat
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別名
- CTCL tumor antigen se20 9 antibody
- LOSK antibody
- se20 9 antibody
see all
画像
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Western blot - Anti-SLK antibody [EPR25108-252] (ab302536)All lanes : Esophagus (Human) Nuclear Lysate - diabetes (ab30236) at 1/1000 dilution
Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting SLK, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 143 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Unknown bands around 140 kDa, 40 kDa and 26 kDa are obaserved. -
![Western blot - Anti-SLK antibody [EPR25108-252] (AB302536)](https://www.abcam.co.jp/ps/products/302/ab302536/Images/AB302536-3-anti-slk-antibody-epr25108252-western-blot-hela-human-cervical-adenocarcinoma-epithelial-cell-whole-cell-lysate.jpg)
Western blot - Anti-SLK antibody [EPR25108-252] (AB302536)All lanes : Anti-SLK antibody [EPR25108-252] (ab302536) at 1/1000 dilution
Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 143 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Unknown bands around 140 kDa, 40 kDa and 26 kDa are obaserved. -
![Anti-SLK antibody [EPR25108-252] (AB302536)](https://www.abcam.co.jp/ps/products/302/ab302536/Images/AB302536-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-SLK antibody [EPR25108-252] (AB302536)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab302536 は論文での使用が確認できていません。