Anti-SLK 抗体 (ab65113)
Key features and details
- Rabbit polyclonal to SLK
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SLK antibody
SLK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SLK -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65113の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
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| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).
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| IHC-P |
1/50 - 1/100.
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| 特記事項 |
|---|
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ICC/IF
1/500 - 1/1000. |
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WB
1/500 - 1/1000. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa). |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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関連性
SLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 9748 Human
- SwissProt: Q9H2G2 Human
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別名
- CTCL tumor antigen se20 9 antibody
- LOSK antibody
- se20 9 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLK antibody (ab65113)This image shows human skeletal muscle tissue stained with ab65113 at 1/50 - 1/100 dilution; with and without the immunising peptide.
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Western blot - Anti-SLK antibody (ab65113)All lanes : Anti-SLK antibody (ab65113) at 1/500 dilution
Lane 1 : extracts from COLO205 cells
Lane 2 : extracts from COLO205 cells with immunising peptide
Predicted band size: 143 kDa
Observed band size: 143 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug. -
Immunocytochemistry/ Immunofluorescence - Anti-SLK antibody (ab65113)Immunofluorescence analysis of HepG2 cells, using ab65113 antibody at 1/500-1/1000 dilution
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab65113 は 2 報の論文で使用されています。
- Tranchevent LC et al. Identification of protein features encoded by alternative exons using Exon Ontology. Genome Res 27:1087-1097 (2017). ICC/IF ; Human . PubMed: 28420690
- Müller T et al. ASAP1 promotes tumor cell motility and invasiveness, stimulates metastasis formation in vivo, and correlates with poor survival in colorectal cancer patients. Oncogene : (2010). WB ; Human . PubMed: 20154719