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AB65113

Anti-SLK 抗体

Anti-SLK antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal SLK antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications.

別名を表示する

KIAA0204, STK2, SLK, STE20-like serine/threonine-protein kinase, STE20-like kinase, hSLK, CTCL tumor antigen se20-9, STE20-related serine/threonine-protein kinase, Serine/threonine-protein kinase 2, STE20-related kinase

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SLK antibody (AB65113)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLK antibody (AB65113)

Immunofluorescence analysis of HepG2 cells, using ab65113 antibody at 1/500-1/1000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLK antibody (AB65113)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLK antibody (AB65113)

This image shows human skeletal muscle tissue stained with ab65113 at 1/50 - 1/100 dilution; with and without the immunising peptide.

Western blot - Anti-SLK antibody (AB65113)
  • WB

Unknown

Western blot - Anti-SLK antibody (AB65113)

The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

All lanes:

Western blot - Anti-SLK antibody (ab65113) at 1/500 dilution

Lane 1:

extracts from COLO205 cells

Lane 2:

extracts from COLO205 cells with immunising peptide

Predicted band size: 143 kDa

Observed band size: 143 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SLK also known as Ste20-like kinase or STLK3 is a serine/threonine-protein kinase. It shows mechanical activity through phosphorylation of specific substrates facilitating cellular processes like migration and proliferation. SLK has an approximate mass of 140 kDa and predominantly occurs in various tissues including epithelial and neural tissues. Its expression is regulated by multiple factors contributing to its dynamic functional role in cells.
Biological function summary

SLK plays an essential role in regulating cytoskeletal organization and cell motility allowing rearrangement of actin fibers. It operates as part of a complex involved in signal transduction cascades that influence cellular shape and movement. This kinase modulates pathways important for cell cycle progression and apoptosis affecting cellular dynamics and integrity.

Pathways

SLK is integral to both the Rho and MAPK signaling pathways which are fundamental for cellular stress responses and growth signals. Within these pathways SLK interacts with proteins such as MINK1 and TAO1 contributing to the coordination of cellular responses to environmental changes and stress signals. SLK's role supports critical intracellular communications that decide cellular fate and adaptation processes.

SLK associates with cancer particularly in cases involving abnormal cell migration and metastasis. It interacts with proteins like the focal adhesion kinase (FAK) which strengthens its participation in oncogenic pathways. SLK also has links to neurodegenerative diseases where its deregulation affects cell survival and death pathways influencing disease progression due to its involvement with proteins like caspase-3 in apoptotic processes.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Mediates apoptosis and actin stress fiber dissolution.
See full target information SLK
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Genome research 27:1087-1097 PubMed28420690

2017

Identification of protein features encoded by alternative exons using Exon Ontology.

Applications

ICC/IF

Species

Human

Léon-Charles Tranchevent,Fabien Aubé,Louis Dulaurier,Clara Benoit-Pilven,Amandine Rey,Arnaud Poret,Emilie Chautard,Hussein Mortada,François-Olivier Desmet,Fatima Zahra Chakrama,Maira Alejandra Moreno-Garcia,Evelyne Goillot,Stéphane Janczarski,Franck Mortreux,Cyril F Bourgeois,Didier Auboeuf

Oncogene 29:2393-403 PubMed20154719

2010

ASAP1 promotes tumor cell motility and invasiveness, stimulates metastasis formation in vivo, and correlates with poor survival in colorectal cancer patients.

Applications

WB

Species

Human

T Müller,U Stein,A Poletti,L Garzia,M Rothley,D Plaumann,W Thiele,M Bauer,A Galasso,P Schlag,M Pankratz,M Zollo,J P Sleeman
View all publications
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