Anti-Slit1 抗体 (ab10984)
Key features and details
- Rabbit polyclonal to Slit1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Slit1 antibody
Slit1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Slit1 -
由来種
Rabbit -
特異性
This affinity purified antibody is directed against mouse SLIT-1 protein. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-1 from human, chicken and frog sources, as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). Cross reactivity with SLIT-1 homologues from other sources has not been determined.
The smaller strong bands observed in the WB shown are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).
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アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Mouse Slit1 aa 450-550 conjugated to keyhole limpet haemocyanin.
Database link: Q80TR4 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This affinity purified antibody is directed against mouse SLIT-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10984の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
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| WB |
1/500. Detects a band of approximately 168 kDa (predicted molecular weight: 168 kDa).
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| 特記事項 |
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WB
1/500. Detects a band of approximately 168 kDa (predicted molecular weight: 168 kDa). |
ターゲット情報
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機能
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. -
組織特異性
Predominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney. -
配列類似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 9 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 20562 Mouse
- Entrez Gene: 65047 Rat
- SwissProt: Q80TR4 Mouse
- SwissProt: O88279 Rat
- Unigene: 40322 Mouse
- Unigene: 30002 Rat
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別名
- KIAA0813 antibody
- MEGF 4 antibody
- MEGF4 antibody
see all
画像
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Western blot - Anti-Slit1 antibody (ab10984)
ab10984-Rabbit polyclonal to Slit1 (1/500)
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000); 20µg of cell lysate per lane. Expected molecular weight: 168 kDa; Exposure time: 5 min
Lane 1: Rat Brain tissue lysate
Lane 2: Mouse Brain tissue LysateNote: The smaller strong bands observed in this WB are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).
ab10984-Rabbit polyclonal to Slit1 (1/500) Secondary ab: Goat anti-rabbit HRP ab6721 (1/5000); 20µg of cell lysate per lane. Expected molecular weight: 168 kDa; Exposure time: 5 min Lane 1: Rat Brain tissue lysate Lane 2: Mouse Brain tissue Lysate Note: The smaller strong bands observed in this WB are likely to be cleavage products. A number of
データシートおよび資料
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Datasheet download
参考文献 (0)
ab10984 は論文での使用が確認できていません。
