JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB10984

Anti-Slit1 抗体

Anti-Slit1 antibody

1

(1 Review)

|

(1 Publication)

Rabbit Polyclonal SLIT1 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Slit1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

Kiaa0813, Slit1, Slit homolog 1 protein, Slit-1

1 Images
Western blot - Anti-Slit1 antibody (AB10984)
  • WB

Unknown

Western blot - Anti-Slit1 antibody (AB10984)

ab10984-Rabbit polyclonal to Slit1 (1/500)

Secondary ab : Goat anti-rabbit IgG ab6721 (1/5000); 20μg of cell lysate per lane. Expected molecular weight : 168 kDa; Exposure time : 5 min

Lane 1 : Rat Brain tissue lysate
Lane 2 : Mouse Brain tissue Lysate

Note : The smaller strong bands observed in this WB are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).

ab10984-Rabbit polyclonal to Slit1 (1/500) Secondary ab : Goat anti-rabbit HRP ab6721 (1/5000); 20μg of cell lysate per lane. Expected molecular weight : 168 kDa; Exposure time : 5 min Lane 1 : Rat Brain tissue lysate Lane 2 : Mouse Brain tissue Lysate Note : The smaller strong bands observed in this WB are likely to be cleavage products. A number of

All lanes:

Western blot - Anti-Slit1 antibody (ab10984)

Predicted band size: 168 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Mouse Slit1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q80TR4

特異性

The smaller strong bands observed in the WB shown are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).

This affinity purified antibody is directed against mouse SLIT-1 protein. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-1 from human, chicken and frog sources, as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). Cross reactivity with SLIT-1 homologues from other sources has not been determined.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
精製に関する特記事項
This affinity purified antibody is directed against mouse SLIT-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
バッファー組成
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Slit1 also known as Slit homolog 1 protein is a large extracellular matrix protein with a mass of approximately 200 kDa. This protein is expressed in the developing and adult nervous system particularly in the brain. It contributes to axon guidance and cellular migration through its interaction with Robo receptors. By serving as a secreted ligand Slit1 plays a critical role in nervous system development guiding neurons to their correct positions.
Biological function summary

Slit1 facilitates the formation of neural circuits by promoting axonal repulsion during neural development. It interacts as part of a receptor-ligand complex with Robo receptors on the cell surface which triggers intracellular signaling cascades. Slit1 also influences the shaping of synaptic connections and acts in a chemorepulsive manner ensuring appropriate neural wiring. Its presence aids cellular organization impacting cell division and movement in the nervous system.

Pathways

Slit1 protein is an integral player in the axon guidance and neuronal network formation pathways. In these processes it modulates actin cytoskeleton dynamics and affects growth cone collapse through signaling with proteins like Robo1 and Robo2. The Slit-Robo signaling pathway works to coordinate cell movement and communication within the developing brain aligning with pathways that involve other guidance cues like Netrins and Semaphorins for balanced nervous system organization.

Slit1 has connections to neurological conditions such as schizophrenia and autism spectrum disorders. Its dysfunction can disrupt normal neural pathways contributing to improper brain connectivity and circuit formation. Slit1 interacts with other proteins implicated in these disorders such as Netrin-1 which supports its role in maintaining neural network integrity. Understanding Slit1's involvement provides insights into potential therapeutic targets for treating such developmental and neurological disorders.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
See full target information Slit1
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

GeroScience : PubMed41075110

2025

GHSR suppression in neurons protects against aging-associated metabolic and cognitive impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Hongying Wang,Chia-Shan Wu,Danilo Landrock,Ariel Nevarez,Shanrun Liu,Anna Thalacker-Mercer,Yanfeng Zhang,Jamie I Baum,Sunja Kim,Bingzhong Xue,Yuxiang Sun
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB151724

Anti-Slit1 antibody [EP5797(2)]

RabMAb|Recombinant

Western blot - Anti-Slit1 antibody [EP5797(2)] (AB151724)

  • 0
  • 0
Primary Antibodies

AB183704

Anti-Slit1 + Slit2 antibody [EP5796(N)]

RabMAb

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (AB183704)

  • 0
  • 0

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB199475

Anti-Semaphorin 3A antibody [EPR19367]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3A antibody [EPR19367] (AB199475)

  • 4
  • 2
Primary Antibodies

AB189491

Anti-Heme Oxygenase 1 antibody [EPR18161-128]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (AB189491)

  • 5
  • 2

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com