Anti-SLD5 抗体 [EPR9318] (ab139683)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9318] to SLD5
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-SLD5 antibody [EPR9318]
SLD5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9318] to SLD5 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human SLD5 aa 1-100. The exact sequence is proprietary.
-
ポジティブ・コントロール
- 293T cells; 293T, PC3, Jurkat and C6 cell lysates.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR9318 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab139683の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
|
特記事項 |
---|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
ターゲット情報
-
機能
The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA. -
配列類似性
Belongs to the GINS4/SLD5 family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 84296 Human
- Entrez Gene: 109145 Mouse
- Entrez Gene: 290842 Rat
- Omim: 610611 Human
- SwissProt: Q9BRT9 Human
- SwissProt: Q99LZ3 Mouse
- SwissProt: Q499W2 Rat
- Unigene: 656996 Human
see all -
別名
- DNA replication complex GINS protein SLD5 antibody
- GINS complex subunit 4 (Sld5 homolog) antibody
- GINS complex subunit 4 antibody
see all
画像
-
Intracellular flow cytometric analysis of permeabilized 293T cells labeling SLD5 with ab139683 at 1/100 dilution (red). Rabbit IgG negative control (green).
-
All lanes : Anti-SLD5 antibody [EPR9318] (ab139683) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : PC3 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab139683 は 4 報の論文で使用されています。
- Bu F et al. Expression Profile of GINS Complex Predicts the Prognosis of Pancreatic Cancer Patients. Onco Targets Ther 13:11433-11444 (2020). PubMed: 33192076
- Köhler K et al. The Cdk8/19-cyclin C transcription regulator functions in genome replication through metazoan Sld7. PLoS Biol 17:e2006767 (2019). PubMed: 30695077
- Wu R et al. H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication. Nucleic Acids Res 45:169-180 (2017). WB . PubMed: 27679476
- Cottineau J et al. Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. J Clin Invest 127:1991-2006 (2017). PubMed: 28414293