Anti-SLC4A4/NBC 抗体 (ab187511)
Key features and details
- Rabbit polyclonal to SLC4A4/NBC
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SLC4A4/NBC antibody
SLC4A4/NBC 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SLC4A4/NBC -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human SLC4A4/NBC aa 1-240 (N terminal).
Database link: Q9Y6R1 -
ポジティブ･コントロール
- PC3 and A549 cell extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.05% Proclin 300
Constituents: 49.95% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187511の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 121 kDa.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/500 - 1/2000. Predicted molecular weight: 121 kDa. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. -
組織特異性
Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules. -
関連疾患
Defects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17). -
配列類似性
Belongs to the anion exchanger (TC 2.A.31) family. -
翻訳後修飾
Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
N-glycosylation is not necessary for the transporter basic functions. -
細胞内局在
Basolateral cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8671 Human
- Entrez Gene: 54403 Mouse
- Entrez Gene: 84484 Rat
- Omim: 603345 Human
- SwissProt: Q9Y6R1 Human
- SwissProt: O88343 Mouse
- SwissProt: Q9JI66 Rat
- Unigene: 5462 Human
see all -
別名
- DKFZp781H1314 antibody
- Electrogenic sodium bicarbonate cotransporter 1 antibody
- hhNMC antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (ab187511)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
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Western blot - Anti-SLC4A4/NBC antibody (ab187511)All lanes : Anti-SLC4A4/NBC antibody (ab187511) at 1/500 dilution
Lane 1 : PC3 cell extract
Lane 2 : A549 cell extract
Developed using the ECL technique.
Predicted band size: 121 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (ab187511)Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling SLC4A4/NBC with ab187511 at 1:100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (ab187511)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (ab187511)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A4/NBC antibody (ab187511)Immunohistochemical analysis of paraffin embedded rat kidney tissue labeling SLC4A4/NBC with ab187511 at 1:100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab187511 は 12 報の論文で使用されています。
- Zhao Q et al. Identification and verification of a prognostic signature based on a miRNA-mRNA interaction pattern in colon adenocarcinoma. Front Cell Dev Biol 11:1161667 (2023). PubMed: 37745305
- Pethe A et al. K+/Cl- cotransporter 2 (KCC2) and Na+/HCO3- cotransporter 1 (NBCe1) interaction modulates profile of KCC2 phosphorylation. Front Cell Neurosci 17:1253424 (2023). PubMed: 37881493
- Cappellesso F et al. Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer. Nat Cancer 3:1464-1483 (2022). PubMed: 36522548
- Huang F et al. SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas. J Cancer 12:5439-5453 (2021). PubMed: 34405007
- Liang Y et al. Identification and Validation of a Novel Inflammatory Response-Related Gene Signature for the Prognosis of Colon Cancer. J Inflamm Res 14:3809-3821 (2021). PubMed: 34408464