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Anti-SLC39A14/ZIP-14 抗体 (ab106568)

Submit a review Q&A (2)References (5)
Western blot - Anti-SLC39A14/ZIP-14 antibody (ab106568)

    Key features and details

    • Rabbit polyclonal to SLC39A14/ZIP-14
    • Suitable for: WB, IHC-P
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-SLC39A14/ZIP-14 antibody
      SLC39A14/ZIP-14 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to SLC39A14/ZIP-14

    • 由来種

      Rabbit

    • 特異性

      At least three isoforms of SLC39A14/ZIP-14 are known to exist; this antibody will detect all isoforms.

    • アプリケーション

      適用あり: WB, IHC-Pmore details

    • 種交差性

      交差種: Mouse, Rat, Human

    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab106568の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 54 kDa.
    IHC-P
    Use a concentration of 1 - 2 µg/ml.
    特記事項
    WB
    Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 54 kDa.
    IHC-P
    Use a concentration of 1 - 2 µg/ml.

    ターゲット情報

    • 機能

      May be able to transport iron (By similarity). Acts as a zinc-influx transporter.

    • 組織特異性

      Ubiquitously expressed, with increased expression in liver, pancreas, fetal liver, thyroid gland, left and right ventricle, right atrium and fetal heart. Weakly expressed in spleen, thymus, and peripheral blood leukocytes.

    • 配列類似性

      Belongs to the ZIP transporter (TC 2.A.5) family.

    • 細胞内局在

      Cell membrane. Cell projection > lamellipodium. Localized to the plasma membrane and also found colocalized with F-actin concentrated on lamellipodiae.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Cig19 antibody
        • KIAA0062 antibody
        • LIV-1 subfamily of ZIP zinc transporter 4 antibody
        • LZT-Hs4 antibody
        • NET34 antibody
        • S39AE_HUMAN antibody
        • SLC39A14 antibody
        • Solute carrier family 39 (zinc transporter), member 14 antibody
        • Solute carrier family 39 member 14 antibody
        • Zinc transporter ZIP14 antibody
        • ZIP-14 antibody
        • ZIP14 antibody
        • Zrt Irt like protein 14 antibody
        • Zrt- and Irt-like protein 14 antibody
        see all

      画像

      • Western blot - Anti-SLC39A14/ZIP-14 antibody (ab106568)
        Western blot - Anti-SLC39A14/ZIP-14 antibody (ab106568)
        All lanes : Anti-SLC39A14/ZIP-14 antibody (ab106568)

        Lane 1 : Wild-type HEK-293T cell lysate
        Lane 2 : SLC39A14 knockout HEK-293T cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : Jurkat cell lysate

        Lysates/proteins at 20 µg per lane.

        Performed under reducing conditions.

        Predicted band size: 54 kDa
        Observed band size: 68 kDa why is the actual band size different from the predicted?



        False colour image of Western blot: Anti-SLC39A14/ZIP-14 antibody staining at 1 μg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab106568 was shown to bind specifically to SLC39A14/ZIP-14. A band was observed at 68 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SLC39A14 knockout cell line ab266126 (knockout cell lysate ab258683). To generate this image, wild-type and SLC39A14 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween®20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

      参考文献 (5)

      ab106568 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab106568 は 5 報の論文で使用されています。

      • Chen Z  et al. Zinc ameliorates human aortic valve calcification through GPR39 mediated ERK1/2 signalling pathway. Cardiovasc Res 117:820-835 (2021). PubMed: 32259211
      • Routhe LJ  et al. Astrocytic expression of ZIP14 (SLC39A14) is part of the inflammatory reaction in chronic neurodegeneration with iron overload. Glia 68:1810-1823 (2020). PubMed: 32077535
      • Liu X  et al. Lysosomal dysfunction is associated with persistent lung injury in dams caused by pregnancy exposure to carbon black nanoparticles. Life Sci 233:116741 (2019). PubMed: 31398419
      • Wongjaikam S  et al. Restoring the impaired cardiac calcium homeostasis and cardiac function in iron overload rats by the combined deferiprone and N-acetyl cysteine. Sci Rep 7:44460 (2017). PubMed: 28287621
      • Tuschl K  et al. Mutations in SLC39A14 disrupt manganese homeostasis and cause childhood-onset parkinsonism-dystonia. Nat Commun 7:11601 (2016). IHC . PubMed: 27231142

      レビューと Q&A

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      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Question

      We aim to purchase SLC39A14 antibody for western blotting as well as ICC. As a part of our project its important for us to know the position of epitop in the protein structure.

      I would be really grateful if you kindly help us in this respect.

      Read More
      Answer

      The peptide used to raise this antibody has been taken from within the region 241-300 of Human SLC39A14, accession number NP_001128625.

      Read More

      Question

      Dear ,
      Would it be possible to try a different antibody against SLC39A14 instead as a replacement. According to your website and the data sheet Anti-SLC39A14 antibody ab106568 should also work for western blotting so could I try this one instead?
      Our delivery address is:

      Read More
      Answer

      As requested, I have issued a free of charge replacement with the order number XXXXX for the antibody ab106568.

      To check the status of the order please contact our Customer Service team and reference this number. You will soon receive a confirmation informing about the delivery date.

      I hope this new vial works as expected. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      Read More

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      For licensing inquiries, please contact partnerships@abcam.com