Anti-SLC34A2 antibody [EPR30528-645]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SLC34A2 antibody [EPR30528-645] (AB325747)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human endometrium tissue staining SLC34A2 with ab325747 at a 1 : 500 (0.99 ug/ml) dilution, ab40772 anti-E Cadherin used at 1 : 1000 (0.325 ug/ml) dilution and ab255609 anti-CD10 used at a 1 : 1000 (0.615 ug/ml) dilution.

Panel A : merged staining of anti-SLC34A2 (green; Opal™520), anti-E Cadherin (magenta; Opal™570) and anti-CD10 (gray; Opal™690) on human endometrium.
Panel B : anti-SLC34A2 staining apical cell membrane of glandular cells in human endometrium.
Panel C : anti-E Cadherin staining glandular cells in human endometrium.
Panel D : anti-CD10 staining stromal cells in human endometrium.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325747, ab40772 and ab255609 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SLC34A2 antibody [EPR30528-645] (AB325747)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human endometrial cancer tissue staining SLC34A2 with ab325747 at a 1 : 500 (0.99 ug/ml) dilution, ab40772 anti-E Cadherin used at 1 : 1000 (0.325 ug/ml) dilution and ab255609 anti-CD10 used at a 1 : 1000 (0.615 ug/ml) dilution.

Panel A : merged staining of anti-SLC34A2 (green; Opal™520), anti-E Cadherin (magenta; Opal™570) and anti-CD10 (gray; Opal™690) on human endometrial cancer.
Panel B : anti-SLC34A2 staining apical cell membrane of glandular cells in human endometrial cancer.
Panel C : anti-E Cadherin staining glandular cells in human endometrial cancer.
Panel D : anti-CD10 staining stromal cells in human endometrial cancer.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325747, ab40772 and ab255609 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [EPR30528-645] (AB325747)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling SLC34A2 with ab325747 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human lung carcinoma. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [EPR30528-645] (AB325747)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling SLC34A2 with ab325747 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human lung. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [EPR30528-645] (AB325747)

Immunohistochemical analysis of paraffin-embedded Clear cell carcinoma of human kidney tissue labeling SLC34A2 with ab325747 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on clear cell carcinoma of human kidney. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [EPR30528-645] (AB325747)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SLC34A2 with ab325747 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human ovarian carcinoma. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [EPR30528-645] (AB325747)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SLC34A2 with ab325747 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on apical cell membrane of human breast. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-SLC34A2 antibody [EPR30528-645] (AB325747)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HepG2, U-2 OS, SUM149PT.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-SLC34A2 antibody [EPR30528-645] (ab325747) at 1/1000 dilution

Lane 1:

NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SUM149PT (human breast adenocarcinoma cell) whole cell lysate at 20 µg

Lane 4:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75-100 kDa,36 kDa

false

Exposure time: 48s

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [EPR30528-645] (AB325747)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling SLC34A2 with ab325747 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human liver. The primary antibody was incubated for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-SLC34A2 antibody [EPR30528-645] (AB325747)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human SLC34A1 or SLC34A3 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (75-100KDa).

All lanes:

Western blot - Anti-SLC34A2 antibody [EPR30528-645] (ab325747) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 2:

293T cells transfected with human SLC34A2 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 3:

293T cells transfected with human SLC34A1 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 4:

293T cells transfected with human SLC34A3 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75-150 kDa,36 kDa,75-100 kDa

false

Exposure time: 15s