Anti-SLC32A1/VGAT 抗体 (ab235952)
Key features and details
- Rabbit polyclonal to SLC32A1/VGAT
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SLC32A1/VGAT antibody
SLC32A1/VGAT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SLC32A1/VGAT -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cynomolgus monkey -
免疫原
Recombinant fragment corresponding to Human SLC32A1/VGAT aa 1-150.
Database link: Q9H598 -
ポジティブ・コントロール
- WB: Mouse brain tissue lysate. IHC-P: Human eyes tissue. ICC/IF: HepG2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purity >95%. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab235952の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/5000.
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
|
特記事項 |
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WB
1/500 - 1/5000. |
ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
ターゲット情報
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機能
Involved in the uptake of GABA and glycine into the synaptic vesicles. -
組織特異性
Retina. Expressed throughout the horizontal cells or more specifically at the terminals. -
配列類似性
Belongs to the amino acid/polyamine transporter 2 family. -
細胞内局在
Cytoplasmic vesicle membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 140679 Human
- Entrez Gene: 22348 Mouse
- Entrez Gene: 83612 Rat
- Omim: 616440 Human
- SwissProt: Q95KE2 Cynomolgus monkey
- SwissProt: Q9H598 Human
- SwissProt: O35633 Mouse
- SwissProt: O35458 Rat
see all -
別名
- bA122O1.1 antibody
- GABA and glycine transporter antibody
- hVIAAT antibody
see all
画像
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Anti-SLC32A1/VGAT antibody (ab235952) at 1/500 dilution + Mouse brain tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC32A1/VGAT antibody (ab235952)
Paraffin-embedded human eye tissue stained for SLC32A1/VGAT using ab235952 at 1/100 dilution in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells labeling SLC32A1/VGAT (green) using ab235952 at 1/100 in ICC/IF. Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab235952 は 1 報の論文で使用されています。
- Xie D et al. TET3 epigenetically controls feeding and stress response behaviors via AGRP neurons. J Clin Invest 132:N/A (2022). PubMed: 36189793