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Anti-SLC27A5/BAL 抗体 (ab74869)

Submit a review Q&A (1)References (2)
Western blot - Anti-SLC27A5/BAL antibody (ab74869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A5/BAL antibody (ab74869)
  • Immunocytochemistry/ Immunofluorescence - Anti-SLC27A5/BAL antibody (ab74869)

Key features and details

  • Rabbit polyclonal to SLC27A5/BAL
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SLC27A5/BAL antibody
    SLC27A5/BAL 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to SLC27A5/BAL

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: human liver carcinoma tissue. WB: extracts from HepG2 cells. ICC/IF: A549 cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab74869の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/1000. Predicted molecular weight: 75 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/1000.
特記事項
WB
1/500 - 1/1000. Predicted molecular weight: 75 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/1000.

ターゲット情報

  • 機能

    Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.

  • 組織特異性

    Predominantly expressed in liver.

  • 配列類似性

    Belongs to the ATP-dependent AMP-binding enzyme family.

  • 細胞内局在

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ACSB antibody
      • ACSVL6 antibody
      • BA CoA ligase antibody
      • BA-CoA ligase antibody
      • BACS antibody
      • BAL antibody
      • Bile acid CoA ligase antibody
      • Bile acid-CoA ligase antibody
      • Bile acyl CoA synthetase antibody
      • Bile acyl-CoA synthetase antibody
      • Cholate CoA ligase antibody
      • Cholate--CoA ligase antibody
      • FACVL3 antibody
      • FATP 5 antibody
      • FATP-5 antibody
      • FATP5 antibody
      • Fatty acid Coenzyme A ligase very long chain 3 antibody
      • Fatty acid transport protein 5 antibody
      • Fatty-acid-coenzyme A ligase antibody
      • FLJ22987 antibody
      • S27A5_HUMAN antibody
      • Slc27a5 antibody
      • Solute carrier family 27 (fatty acid transporter) member 5 antibody
      • Solute carrier family 27 member 5 antibody
      • Very long chain acyl CoA synthetase homolog 2 antibody
      • Very long chain acyl CoA synthetase related protein antibody
      • very long-chain 3 antibody
      • Very long-chain acyl-CoA synthetase homolog 2 antibody
      • Very long-chain acyl-CoA synthetase-related protein antibody
      • VLACS related antibody
      • VLACS-related antibody
      • VLACSR antibody
      • VLCS H2 antibody
      • VLCS-H2 antibody
      • VLCSH2 antibody
      see all

    画像

    • Western blot - Anti-SLC27A5/BAL antibody (ab74869)
      Western blot - Anti-SLC27A5/BAL antibody (ab74869)
      All lanes : Anti-SLC27A5/BAL antibody (ab74869) at 1/500 dilution

      Lane 1 : Extracts from HepG2 cells
      Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 75 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A5/BAL antibody (ab74869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A5/BAL antibody (ab74869)

      Immunohistochemistry analysis of SLC27A5/BAL in paraffin-embedded human liver carcinoma tissue using ab74869, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

    • Immunocytochemistry/ Immunofluorescence - Anti-SLC27A5/BAL antibody (ab74869)
      Immunocytochemistry/ Immunofluorescence - Anti-SLC27A5/BAL antibody (ab74869)

      Immunofluorescence analysis of SLC27A5/BAL in A549 cells, using ab74869, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

    参考文献 (2)

    ab74869 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab74869 は 2 報の論文で使用されています。

    • Zhang F  et al. Identifying SLC27A5 as a potential prognostic marker of hepatocellular carcinoma by weighted gene co-expression network analysis and in vitro assays. Cancer Cell Int 21:174 (2021). PubMed: 33731144
    • Przybylla S  et al. Analysis of the Bile Salt Export Pump (ABCB11) Interactome Employing Complementary Approaches. PLoS One 11:e0159778 (2016). IP ; Human . PubMed: 27472061

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Could this antibody cross-react with mouse samples?

    Read More
    Answer

    Thank you very much for your call yesterday and for your interest in ab74869.

    I've heard back from the lab, and unfortunately the immunogen of this antibody only shares 57% identity with the mouse sequence, so it's not highly likely to cross-react. The immunogens of the other SLC27A5 antibodies have higher identity with mouse, however neither is high enough to definitely say there will be cross-reaction:

    Ab97884: 78%
    Ab89008: 72%

    If you'd like to go ahead and try one of these, we do have a testing program that you can take advantage of, since we can't guarantee that any of these will work with mouse samples.

    I hope this information will be useful, but if you have further questions or need anything else, please let me know and I'll be happy to help.

    Read More

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