Anti-SLC25A13/Citrin 抗体 [EPR9969(B)] (ab167166)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9969(B)] to SLC25A13/Citrin
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SLC25A13/Citrin antibody [EPR9969(B)]
SLC25A13/Citrin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9969(B)] to SLC25A13/Citrin -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human SLC25A13/Citrin (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HeLa, Hap1, HepG2 and SH-SY5Y cell lysates; Human fetal liver tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9969(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167166の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 70 kDa. |
ターゲット情報
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機能
Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle. -
組織特異性
High levels in liver and low levels in kidney, pancreas, placenta, heart and brain. -
関連疾患
Defects in SLC25A13 are the cause of citrullinemia type 2 (CTLN2) [MIM:603471]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN2 is characterized by neuropsychiatric symptoms including abnormal behaviors, loss of memory, seizures and coma. Death can result from brain edema. Onset is sudden and usually between the ages of 20 and 50 years.
Defects in SLC25A13 are the cause of neonatal intrahepatic cholestasis due to citrin deficiency (NICCD) [MIM:605814]. NICCD is a form of citrullinemia type 2 with neonatal onset. NICCD is characterized by suppression of the bile flow, hepatic fibrosis, low birth weight, growth retardation, hypoproteinemia, variable liver dysfunction. NICCD is generally not severe and symptoms disappear by one year of age with an appropriate diet. Years or even decades later, however, some individuals develop the characteristic features of citrullinemia type 2 with neuropsychiatric symptoms. -
配列類似性
Belongs to the mitochondrial carrier family.
Contains 4 EF-hand domains.
Contains 3 Solcar repeats. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10165 Human
- Omim: 603859 Human
- SwissProt: Q9UJS0 Human
- Unigene: 489190 Human
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別名
- AI785475 antibody
- ARALAR2 antibody
- Calcium binding mitochondrial carrier protein Aralar2 antibody
see all
画像
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All lanes : Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SLC25A13 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDaLanes 1- 2: Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab167166 was shown to react with SLC25A13 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265668 (knockout cell lysate ab258192) was used. Wild-type HeLa and SLC25A13 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab167166 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/500 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : SLC25A13 knockout HAP1 cell lysate
Lane 3 : SH-5YSY cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 70 kDaLanes 1 - 3: Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab167166 was shown to recognize SLC25A13 when SLC25A13 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A13 knockout samples were subjected to SDS-PAGE. ab167166 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/1000 dilution
Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 cell lysates
Lane 3 : SH-SY5Y cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 70 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab167166 は論文での使用が確認できていません。