AB200201

Anti-SLC25A12 抗体 [EPR16294]

Name: Anti-SLC25A12 抗体 [EPR16294] (ab200201) | Abcam
Brand: Abcam Limited
SKU: AB200201
Price: 45000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-SLC25A12 antibody [EPR16294]

RabMAb
Recombinant
KO Validated
詳細を見る

(1 Review)

(7 Publications)

Rabbit Recombinant Monoclonal SLC25A12 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

別名を表示する

AGC1, ARALAR1, SLC25A12, Araceli hiperlarga, Mitochondrial aspartate glutamate carrier 1, Solute carrier family 25 member 12, Aralar, Aralar1

6 Images

Supplier Data

Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] (AB200201)

SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution.

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Jurkat whole cell lysate 10 μg (Input).

Lane 2 : ab200201 IP in Jurkat whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 second.

All lanes:

Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] (ab200201)

Predicted band size: 74 kDa

false

Supplier Data

Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] (AB200201)

SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution (Panel A) or ab192244 at 1/1000 dilution (Panel B).

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Jurkat whole cell lysate 10 μg (Input).

Lane 2 : ab200201 IP in Jurkat whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time (Panel A and Panel B) : 30 second.

All lanes:

Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] (ab200201)

Predicted band size: 74 kDa

false

Lab

Western blot - Anti-SLC25A12 antibody [EPR16294] (AB200201)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : SLC25A12 knockout HAP1 cell lysate (20 μg)
Lane 3 : Human skeletal muscle lysate (20 μg)
Lane 4 : A431 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab200201 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab200201 was shown to recognize SLC25A12 when SLC25A12 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A12 knockout samples were subjected to SDS-PAGE. ab200201 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 andGoat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SLC25A12 antibody [EPR16294] (ab200201)

Predicted band size: 74 kDa

Observed band size: 72 kDa

false

Supplier Data

Western blot - Anti-SLC25A12 antibody [EPR16294] (AB200201)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 74 kDa

Observed band size: 75 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-SLC25A12 antibody [EPR16294] (AB200201)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/5000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 74 kDa

Observed band size: 75 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-SLC25A12 antibody [EPR16294] (AB200201)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat heart lysate at 10 µg

Lane 6:

Rat kidney lysate at 10 µg

Lane 7:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 8:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 9:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 10:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 74 kDa

Observed band size: 75 kDa

false

Exposure time: 15s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16294

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SLC25A12 also known as the mitochondrial aspartate/glutamate carrier 1 (AGC1) functions as a transporter protein. It facilitates the exchange of aspartate and glutamate across the inner mitochondrial membrane. This protein has a molecular mass of approximately 77 kDa. SLC25A12 is mainly found in tissues with high metabolic activity including the brain heart and skeletal muscle.

Biological function summary

SLC25A12 plays an important role in energy metabolism. It is involved in transferring aspartate from the mitochondria to the cytosol and glutamate in the opposite direction which is essential for the malate-aspartate shuttle. This carrier contributes significantly to maintaining the balance of cellular metabolism by linking the citric acid cycle and amino acid synthesis to mitochondrial energy production. SLC25A12 operates as part of a larger complex of mitochondrial transport proteins which facilitate similar exchanges.

Pathways

SLC25A12 is vitally linked to the malate-aspartate shuttle and the urea cycle. In the malate-aspartate shuttle it works closely with other proteins including SLC25A13 (AGC2) to maintain NADH/NAD+ balance across the mitochondrial membrane. This balance is important for continuous ATP production during oxidative phosphorylation. The function of SLC25A12 in these pathways highlights its significance in both energy metabolism and nitrogen disposal processes interlinking with proteins involved in these metabolic pathways.

Mutations and altered expression of SLC25A12 have been associated with neurological conditions such as autism and epilepsy. Research shows a correlation between SLC25A12 dysfunction and disrupted energy metabolism in neurons which may contribute to the pathophysiology of these conditions. Furthermore interaction with proteins like SLC25A13 has been observed in some metabolic disorders emphasizing the interconnected nature of metabolic pathways and their role in disease.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle (PubMed : 11566871, PubMed : 19641205, PubMed : 24515575). Also mediates the uptake of L-cysteinesulfinate by mitochondria in exchange of L-glutamate and proton. Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation (PubMed : 11566871).

See full target information SLC25A12

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 90:102047 PubMed39419476

2024

Distinct roles for the domains of the mitochondrial aspartate/glutamate carrier citrin in organellar localization and substrate transport.

Applications

Unspecified application

Species

Unspecified reactive species

Sotiria Tavoulari,Denis Lacabanne,Gonçalo C Pereira,Chancievan Thangaratnarajah,Martin S King,Jiuya He,Suvagata R Chowdhury,Lisa Tilokani,Shane M Palmer,Julien Prudent,John E Walker,Edmund R S Kunji

Nature genetics 56:1614-1623 PubMed38977856

2024

Metabolic gene function discovery platform GeneMAP identifies SLC25A48 as necessary for mitochondrial choline import.

Applications

Unspecified application

Species

Unspecified reactive species

Artem Khan,Gokhan Unlu,Phillip Lin,Yuyang Liu,Ece Kilic,Timothy C Kenny,Kıvanç Birsoy,Eric R Gamazon

Molecular and cellular biology 44:226-244 PubMed38828998

2024

Reduced Protein Import via TIM23 SORT Drives Disease Pathology in TIMM50-Associated Mitochondrial Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan J Crameri,Catherine S Palmer,Tegan Stait,Thomas D Jackson,Matthew Lynch,Adriane Sinclair,Leah E Frajman,Alison G Compton,David Coman,David R Thorburn,Ann E Frazier,Diana Stojanovski

Nature cell biology 25:467-480 PubMed36690850

2023

Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space.

Applications

Unspecified application

Species

Unspecified reactive species

Elias Adriaenssens,Bob Asselbergh,Pablo Rivera-Mejías,Sven Bervoets,Leen Vendredy,Vicky De Winter,Katrien Spaas,Riet de Rycke,Gert van Isterdael,Francis Impens,Thomas Langer,Vincent Timmerman

The Journal of biological chemistry 295:444-457 PubMed31767682

2019

The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Yasukawa,Daisuke Kinoshita,Keisuke Yaku,Takashi Nakagawa,Takumi Koshiba

Journal of Cancer 10:6570-6583 PubMed31777586

2019

Up-regulation of EMT-related gene VCAN by NPM1 mutant-driven TGF-β/cPML signalling promotes leukemia cell invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Yang,Lu Wang,Zailin Yang,Hongjun Jin,Qin Zou,Qian Zhan,Yuting Tang,Yao Tao,Li Lei,Yipei Jing,Xueke Jiang,Ling Zhang

Neurobiology of disease 132:104559 PubMed31376479

2019

Generation of an Atxn2-CAG100 knock-in mouse reveals N-acetylaspartate production deficit due to early Nat8l dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Nesli-Ece Sen,Júlia Canet-Pons,Melanie V Halbach,Aleksandar Arsovic,Ulrich Pilatus,Woon-Hyung Chae,Zeynep-Ece Kaya,Kay Seidel,Ewa Rollmann,Michel Mittelbronn,David Meierhofer,Chris I De Zeeuw,Laurens W J Bosman,Suzana Gispert,Georg Auburger

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC25A12 抗体 [EPR16294]

Anti-SLC25A12 antibody [EPR16294]

Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] (AB200201)

Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] (AB200201)

Western blot - Anti-SLC25A12 antibody [EPR16294] (AB200201)

Western blot - Anti-SLC25A12 antibody [EPR16294] (AB200201)

Western blot - Anti-SLC25A12 antibody [EPR16294] (AB200201)

Western blot - Anti-SLC25A12 antibody [EPR16294] (AB200201)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (7)

代替製品

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise