Anti-SLC22A1/OCT1 抗体 [EPR3668(N)] (ab181022)
![Western blot - Anti-SLC22A1/OCT1 antibody [EPR3668(N)] (ab181022)](https://www.abcam.co.jp/ps/products/181/ab181022/Images/ab181022-213150-anti-slc22a1-antibody-epr3668n-western-blot.jpg "Western blot - Anti-SLC22A1/OCT1 antibody [EPR3668(N)] (ab181022)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3668(N)] to SLC22A1/OCT1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SLC22A1/OCT1 antibody [EPR3668(N)]
SLC22A1/OCT1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3668(N)] to SLC22A1/OCT1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Jurkat, SW480, HepG2 and Caco-2 cell lysate. HepG2 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR3668(N) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181022の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa. |
ターゲット情報
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機能
Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase. -
組織特異性
Widely expressed with high level in liver. Isoform 1 and isoform 2 are expressed in liver. Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in glial cell lines. -
配列類似性
Belongs to the major facilitator superfamily. Organic cation transporter family. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Basolateral cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6580 Human
- Omim: 602607 Human
- SwissProt: O15245 Human
- SwissProt: O15395 Human
- Unigene: 117367 Human
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別名
- hOCT1 antibody
- OCT1 antibody
- oct1_cds antibody
see all
画像
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Western blot - Anti-SLC22A1/OCT1 antibody [EPR3668(N)] (ab181022)All lanes : Anti-SLC22A1/OCT1 antibody [EPR3668(N)] (ab181022) at 1/10000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 61 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Flow Cytometry (Intracellular) - Anti-SLC22A1/OCT1 antibody [EPR3668(N)] (ab181022)](https://www.abcam.co.jp/ps/products/181/ab181022/Images/ab181022-213149-anti-slc22a1-antibody-epr3668n-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SLC22A1/OCT1 antibody [EPR3668(N)] (ab181022)Intracellular Flow Cytometry analysis of HepG2 cells labeling SLC22A1/OCT1 using ab181022 at a 1/10 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
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![Anti-SLC22A1/OCT1 antibody [EPR3668(N)] (ab181022)](https://www.abcam.co.jp/ps/products/181/ab181022/Images/ab181022-3-benefits-of-recombinant-antibodies.png)
Anti-SLC22A1/OCT1 antibody [EPR3668(N)] (ab181022)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (4)
ab181022 は 4 報の論文で使用されています。
- Cai D et al. Identification of the Tumor Immune Microenvironment and Therapeutic Biomarkers by a Novel Molecular Subtype Based on Aging-Related Genes in Hepatocellular Carcinoma. Front Surg 9:836080 (2022). PubMed: 35392063
- Barilli A et al. Organic Cation Transporters (OCTs) in EpiAirway™, A Cellular Model of Normal Human Bronchial Epithelium. Biomedicines 8:N/A (2020). PubMed: 32438722
- Arner P et al. Mapping of biguanide transporters in human fat cells and their impact on lipolysis. Diabetes Obes Metab 20:2416-2425 (2018). PubMed: 29862627
- Thakur S et al. Metformin Targets Mitochondrial Glycerophosphate Dehydrogenase to Control Rate of Oxidative Phosphorylation and Growth of Thyroid Cancer In Vitro and In Vivo. Clin Cancer Res N/A:N/A (2018). PubMed: 29691295