Anti-SLC1A5/ASCT2 抗体 [CAL33] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (AB251591)

Immunofluorescent analysis of 100% methanol fixed Jurkat (human T cell leukemia T lymphocyte) cells labeling SLC1A5/ASCT2 with ab237704 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Jurkat cells. The nuclear counter stain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (AB251591)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for SLC1A5/ASCT2 using ab237704 at 0.5 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (AB251591)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling SLC1A5/ASCT2 with ab237704 at 1/1600 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human lung carcinoma is observed. The section was incubated with ab237704 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (AB251591)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling SLC1A5/ASCT2 with ab237704 at 1/1600 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human colon carcinoma is observed. The section was incubated with ab237704 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (AB251591)

Immunofluorescent analysis of 100% methanol fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling SLC1A5/ASCT2 with ab237704 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in HeLa cells. The nuclear counter stain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (AB251591)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte) cell line labeling SLC1A5/ASCT2 with ab237704 at 1/40 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (AB251591)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling SLC1A5/ASCT2 with ab237704 at 1/400 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

• IP

Lab

Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (AB251591)

SLC1A5/ASCT2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab237704 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237704 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1 : Jurkat whole cell lysate 10 μg (Input).
Lane 2 : ab237704 IP in Jurkat whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab237704 in Jurkat whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

ASCT2 (SLC1A5) can form dimers or trimers based on literature. (PMID : 23756778).

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

All lanes:

Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] (<a href='/products/primary-antibodies/slc1a5-asct2-antibody-cal33-ab237704'>ab237704</a>) at 1/30 dilution

Predicted band size: 56 kDa

false

Exposure time: 3s

• IP

Lab

Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (AB251591)

SLC1A5/ASCT2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab237704 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237704 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).
Lane 2 : ab237704 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab237704 in HeLa whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

ASCT2 (SLC1A5) can form dimers or trimers based on literature. (PMID : 23756778).

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

All lanes:

Immunoprecipitation - Anti-SLC1A5/ASCT2 antibody [CAL33] (<a href='/products/primary-antibodies/slc1a5-asct2-antibody-cal33-ab237704'>ab237704</a>) at 1/30 dilution

Predicted band size: 56 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free (AB251591)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237704).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time :
Lane 1 : 80 seconds
Lane 2 : 40 seconds

An extra band around 50 kDa might be observed. We are not sure how to define it.

All lanes:

Western blot - Anti-SLC1A5/ASCT2 antibody [CAL33] (<a href='/products/primary-antibodies/slc1a5-asct2-antibody-cal33-ab237704'>ab237704</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole lysate at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) whole lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

false