Anti-SLC17A5 抗体 (ab153920)
Key features and details
- Rabbit polyclonal to SLC17A5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SLC17A5 antibody
SLC17A5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SLC17A5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to a region within amino acids 9-242 of Human SLC17A5.
-
ポジティブ･コントロール
- Jurkat whole cell lysate; HepG2 cells; Human BT474 xenograft tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153920の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 54, 31 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 54, 31 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
-
機能
Transports glucuronic acid and free sialic acid out of the lysosome after it is cleaved from sialoglycoconjugates undergoing degradation, this is required for normal CNS myelination. Mediates aspartate and glutamate membrane potential-dependent uptake into synaptic vesicles and synaptic-like microvesicles. Also functions as an electrogenic 2NO(3)(-)/H(+) cotransporter in the plasma membrane of salivary gland acinar cells, mediating the physiological nitrate efflux, 25% of the circulating nitrate ions is typically removed and secreted in saliva. -
組織特異性
Found in fetal lung and small intestine, and at lower level in fetal skin and muscle. In the adult, detected in placenta, kidney and pancreas. Abundant in the endothelial cells of tumors from ovary, colon, breast and lung, but is not detected in endothelial cells from the corresponding normal tissues. -
関連疾患
Salla disease
Infantile sialic acid storage disorder -
配列類似性
Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. -
細胞内局在
Cell membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Lysosome membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 26503 Human
- Omim: 604322 Human
- SwissProt: Q9NRA2 Human
- Unigene: 597422 Human
-
別名
- AST antibody
- H(+)/nitrate cotransporter antibody
- H(+)/sialic acid cotransporter antibody
see all
画像
-
Western blot - Anti-SLC17A5 antibody (ab153920)Anti-SLC17A5 antibody (ab153920) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 54, 31 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC17A5 antibody (ab153920)Immunohistochemical analysis of paraffin-embedded BT474 xenograft labeling SLC17A5 with ab153920 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-SLC17A5 antibody (ab153920)Immunofluorescent analysis of methanol-fixed HepG2 cells labeling SLC17A5 with ab153920 at 1/500 dilution (green). Blue: Hoechst 33343 staining.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab153920 は論文での使用が確認できていません。