AB191315

Anti-SLC12A1/NKCC2 抗体 - N-terminal

Name: Anti-SLC12A1/NKCC2 抗体 - N-terminal (ab191315) | Abcam
Brand: Abcam Limited
SKU: AB191315
Price: 75000 JPY
Availability: InStock

Anti-SLC12A1/NKCC2 antibody - N-terminal

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Polyclonal SLC12A1/NKCC2 antibody. N-terminal. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SLC12A1 aa 100-150.

別名を表示する

NKCC2, SLC12A1, Solute carrier family 12 member 1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, Kidney-specific Na-K-Cl symporter, Na-K-2Cl cotransporter 2, BSC1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

Immunohistochemical analysis of paraffin-embedded section of rat kidney tissue, labelling SLC12A1/NKCC2 with ab191315 at 2 μg/ml.

Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

Immunohistochemical analysis of paraffin-embedded section of mouse kidney tissue, labelling SLC12A1/NKCC2 with ab191315 at 2 μg/ml.

Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat

アプリケーション

IHC-P

applications

免疫原

Synthetic Peptide within Human SLC12A1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q13621

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

Constituents: 76.92% Trehalose, 17.31% Sodium chloride, 3.85% Disodium hydrogenorthophosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The SLC12A1 protein also known as the NKCC2 transporter is an important player in ion transport within renal physiology. With a molecular weight of around 120 kDa NKCC2 functions in the reabsorption of sodium potassium and chloride ions a function facilitated by its location in the thick ascending limb of the loop of Henle in the kidneys. This location and functionality are critical for renal concentration mechanisms and contribute to the maintenance of electrolyte and fluid balance.

Biological function summary

This transporter plays an essential role in concentrating urine and regulating blood pressure. NKCC2 is a part of the larger solute carrier family and does not usually form complexes with other proteins. It facilitates the trans-epithelial movement of Na+ K+ and 2Cl- ions from the lumen into renal epithelial cells which is fundamental for the creation of an osmotic gradient essential for water reabsorption.

Pathways

The SLC12A1 protein is a significant component of renal electrolyte transport and blood pressure regulation pathways. It is intricately involved in the renin-angiotensin-aldosterone system (RAAS) which regulates blood volume and systemic vascular resistance. In these pathways NKCC2's activity tightly connects it to other ion channel proteins and transporters such as ENaC which also play roles in blood pressure regulation by influencing sodium handling in the nephron.

Dysregulation of NKCC2 function can result in Bartter syndrome and hypertension. Bartter syndrome a rare disorder characterized by salt wasting and electrolyte imbalances stems from mutations affecting NKCC2. This transporter also impacts hypertension where its dysregulation can alter sodium reabsorption processes linking it to other proteins involved in blood pressure control such as the mineralocorticoid receptor. Understanding the role of NKCC2 provides insight into potential therapeutic targets for these conditions.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation (PubMed : 21321328). Electrically silent transporter system (By similarity).

See full target information SLC12A1

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental nephrology 29:393-404 PubMed39666151

2024

Nephrotic syndrome induces the upregulation of cell proliferation-related genes in tubular cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Suzuki,Ryohei Kaseda,Yusuke Nakagawa,Hirofumi Watanabe,Tadashi Otsuka,Suguru Yamamoto,Yoshikatsu Kaneko,Shin Goto,Taiji Matsusaka,Ichiei Narita

FASEB journal : official publication of the Federa 33:5887-5902 PubMed30721632

2019

IL-6/Smad2 signaling mediates acute kidney injury and regeneration in a murine model of neonatal hyperoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmine Mohr,Jenny Voggel,Christina Vohlen,Katharina Dinger,Claudia Dafinger,Gregor Fink,Heike Göbel,Max C Liebau,Jörg Dötsch,Miguel A Alejandre Alcazar

Dose-response : a publication of International Hormesis Society 16:1559325818811522 PubMed30479587

2018

Sodium Dichloroacetate Pharmacological Effect as Related to Na-K-2Cl Cotransporter Inhibition in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jūratė Stanevičiūtė,Milda Juknevičienė,Jolita Palubinskienė,Ingrida Balnytė,Angelija Valančiūtė,Rūta Vosyliūtė,Kęstutis Sužiedėlis,Vaiva Lesauskaitė,Donatas Stakišaitis

BMC genomics 17:724 PubMed27613513

2016

A missense mutation in solute carrier family 12, member 1 (SLC12A1) causes hydrallantois in Japanese Black cattle.

Applications

IHC-Fr

Species

Cow

Shinji Sasaki,Kiyotoshi Hasegawa,Tomoko Higashi,Yutaka Suzuki,Sumio Sugano,Yasuaki Yasuda,Yoshikazu Sugimoto

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLC12A1/NKCC2 抗体 - N-terminal

Anti-SLC12A1/NKCC2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A1/NKCC2 antibody - N-terminal (AB191315)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise