Anti-SKP2 抗体 (ab68455)
Key features and details
- Rabbit polyclonal to SKP2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SKP2 antibody
SKP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SKP2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human SKP2 aa 2-20 (N terminal). Different from the related rat and mouse sequences by three amino acids.
Sequence:HRKHLQEIPDLSSNVATSF
Database link: Q13309 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68455の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
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| IHC-P |
Use a concentration of 1 - 2 µg/ml.
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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機能
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 F-box domain.
Contains 9 LRR (leucine-rich) repeats. - Information by UniProt
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参照データベース
- Entrez Gene: 6502 Human
- Omim: 601436 Human
- SwissProt: Q13309 Human
- Unigene: 23348 Human
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別名
- CDK2/Cyclin A associated protein p45 antibody
- Cyclin A/CDK2 associated protein p45 antibody
- Cyclin-A/CDK2-associated protein p45 antibody
see all
画像
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Western blot - Anti-SKP2 antibody (ab68455)All lanes : Anti-SKP2 antibody (ab68455) at 1 µg/ml
Lane 1 : Whole cell lysates prepared from Hela cells
Lane 2 : Whole cell lysates prepared from 6T-CEM cells
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size: 48 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKP2 antibody (ab68455)ab68455 at 1µg/ml staining SKP2 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The tissue underwent enzymatic antigen retrieval in pepsin and trypsin. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
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Western blot - Anti-SKP2 antibody (ab68455)All lanes : Anti-SKP2 antibody (ab68455) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Additional bands at: 29 kDa, 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
The predicted molecular weight of SKP2 is 48 kDa (SwissProt), however we expect to observe a banding pattern at 53 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab68455 は 16 報の論文で使用されています。
- Mejía-Hernández JO et al. Targeting MDM4 as a Novel Therapeutic Approach in Prostate Cancer Independent of p53 Status. Cancers (Basel) 14:N/A (2022). PubMed: 36010941
- Liu B et al. SKP2-Promoted Ubiquitination of FOXO3 Promotes the Development of Asthma. J Immunol 206:2366-2375 (2021). PubMed: 33837090
- Zhang X et al. IMP3 accelerates the progression of prostate cancer through inhibiting PTEN expression in a SMURF1-dependent way. J Exp Clin Cancer Res 39:190 (2020). PubMed: 32938489
- Qiu C et al. Bisdemethoxycurcumin Inhibits Hepatocellular Carcinoma Proliferation Through Akt Inactivation via CYLD-Mediated Deubiquitination. Drug Des Devel Ther 14:993-1001 (2020). PubMed: 32184568
- Yang Y et al. Skp2 inhibitor SKPin C1 decreased viability and proliferation of multiple myeloma cells and induced apoptosis. Braz J Med Biol Res 52:e8412 (2019). PubMed: 31038581