Anti-SIRT6 抗体 [EPR5079(N)] (ab176345)
![Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)](https://www.abcam.co.jp/ps/products/176/ab176345/Images/ab176345-388731-anti-sirt6-antibody-epr5079n-western-blot-hela-lysates.jpg "Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5079(N)] to SIRT6
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SIRT6 antibody [EPR5079(N)]
SIRT6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5079(N)] to SIRT6 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: K562, Jurkat, 293T, HepG2 and HeLa whole cell lysate (ab150035).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5079(N) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176345の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa. |
ターゲット情報
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機能
NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. -
配列類似性
Belongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain. -
細胞内局在
Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions. - Information by UniProt
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参照データベース
- Entrez Gene: 51548 Human
- Omim: 606211 Human
- SwissProt: Q8N6T7 Human
- Unigene: 423756 Human
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別名
- 2810449N18Rik antibody
- AI043036 antibody
- Mono ADP ribosyltransferase sirtuin 6 antibody
see all
画像
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Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)All lanes : Anti-SIRT6 antibody [EPR5079(N)] (ab176345) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SIRT6 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Lanes 1-2: Merged signal (red and green). Green - ab176345 observed at 45 kDa. Red - loading control ab8245 observed at 37 kDa.
ab176345 Anti-SIRT6 antibody [EPR5079(N)] was shown to specifically react with SIRT6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265054 (knockout cell lysate ab257673) was used. Wild-type and SIRT6 knockout samples were subjected to SDS-PAGE. ab176345 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)](https://www.abcam.co.jp/ps/products/176/ab176345/Images/ab176345-279559-anti-sirt6-antibody-epr5079n-western-blot.jpg)
Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)All lanes : Anti-SIRT6 antibody [EPR5079(N)] (ab176345) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SIRT6 knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 39 kDaLanes 1 - 3: Merged signal (red and green). Green - ab176345 (unpurified) observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab176345 was shown to specifically recognize SIRT6 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when SIRT6 knockout samples were examined. Wild-type and SIRT6 knockout samples were subjected to SDS-PAGE. Ab176345 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/30,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)](https://www.abcam.co.jp/ps/products/176/ab176345/Images/ab176345-344803-Anti-sirt6-antibody-EPR5079-N-westernblot-human.jpg)
Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)All lanes : Anti-SIRT6 antibody [EPR5079(N)] (ab176345) at 1/1000 dilution (Purified)
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 42-43 kDa why is the actual band size different from the predicted?The doublets detected and observed molecular weights are consistent with what have been described in literatures PMID: 30542728, 29143563, 24169447 and 30670969.
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![Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)](https://www.abcam.co.jp/ps/products/176/ab176345/Images/ab176345-203686-anti-sirt6-antibody-epr5079n-western-blot.jpg)
Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)All lanes : Anti-SIRT6 antibody [EPR5079(N)] (ab176345) at 1/1000 dilution (unpurified)
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa -
![Anti-SIRT6 antibody [EPR5079(N)] (ab176345)](https://www.abcam.co.jp/ps/products/176/ab176345/Images/ab176345-5-benefits-of-recombinant-antibodies.png)
Anti-SIRT6 antibody [EPR5079(N)] (ab176345)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab176345 は 1 報の論文で使用されています。
- Jin Y et al. Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging. Sci Rep 8:5145 (2018). WB . PubMed: 29572511