Anti-SIRT4 抗体 (ab10140)
Key features and details
- Goat polyclonal to SIRT4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SIRT4 antibody
SIRT4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SIRT4 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig -
免疫原
Synthetic peptide:
SRCGELLPLIDPC
, corresponding to C terminal amino acids 302-314 of Human SIRT4. -
ポジティブ・コントロール
- IHC: Human skeletal muscle and pancreas tissue WB: Human kidney and U937 lysate, HEK293 cell lysate
-
特記事項
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10140の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use a concentration of 2 - 3 µg/ml. Steamed antigen retrieval with citrate buffer pH 6 is recommended.
|
特記事項 |
---|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 2 - 3 µg/ml. Steamed antigen retrieval with citrate buffer pH 6 is recommended. |
ターゲット情報
-
機能
Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD(+) levels (PubMed:24043310). Down-regulates insulin secretion. -
組織特異性
Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus. -
配列類似性
Belongs to the sirtuin family. Class II subfamily.
Contains 1 deacetylase sirtuin-type domain. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
-
参照データベース
- Entrez Gene: 519328 Cow
- Entrez Gene: 23409 Human
- Entrez Gene: 75387 Mouse
- Entrez Gene: 100125349 Pig
- Entrez Gene: 304539 Rat
- Omim: 604482 Human
- SwissProt: Q1JQC6 Cow
- SwissProt: Q9Y6E7 Human
see all -
別名
- MGC130046 antibody
- MGC130047 antibody
- MGC57437 antibody
see all
画像
-
Anti-SIRT4 antibody (ab10140) at 1 µg/ml + Human Kidney Lysate (RIPA buffer).35 ug total protein was loaded.
Predicted band size: 36 kDaab10140 staining (1
Incubation with the primary Ab was carried out for 1 hr at room temperature.µ g/ml) of Human Kidney lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (19)
ab10140 は 19 報の論文で使用されています。
- Wei W et al. SIRT4 Is Highly Expressed in Retinal Müller Glial Cells. Front Neurosci 16:840443 (2022). PubMed: 35185463
- Wang Y et al. SIRT4-Catalyzed Deacetylation of Axin1 Modulates the Wnt/β-Catenin Signaling Pathway. Front Oncol 12:872444 (2022). PubMed: 35707358
- Qin M et al. Autophagy in Rat Müller Glial Cells Is Modulated by the Sirtuin 4/AMPK/mTOR Pathway and Induces Apoptosis under Oxidative Stress. Cells 11:N/A (2022). PubMed: 36078054
- Xu L et al. Circular RNA OMA1 regulates the progression of breast cancer via modulation of the miR-1276/SIRT4 axis. Mol Med Rep 24:N/A (2021). PubMed: 34414449
- Du L et al. Loss of SIRT4 promotes the self-renewal of Breast Cancer Stem Cells. Theranostics 10:9458-9476 (2020). PubMed: 32863939