AB10140

Anti-SIRT4 抗体

Anti-SIRT4 antibody

(1 Review)

(19 Publications)

Goat Polyclonal SIRT4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human SIRT4 aa 300 to C-terminus.

別名を表示する

SIR2L4, SIRT4, NAD-dependent ADP-ribosyltransferase sirtuin-4, NAD-dependent protein biotinylase sirtuin-4, NAD-dependent protein deacetylase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4

1 Images

Unknown

Western blot - Anti-SIRT4 antibody (AB10140)

ab10140 staining (1μg/ml) of Human Kidney lysate (RIPA buffer, 35μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Incubation with the primary Ab was carried out for 1 hr at room temperature.

All lanes:

Western blot - Anti-SIRT4 antibody (ab10140) at 1 µg/mL

All lanes:

Human Kidney Lysate (RIPA buffer).35 ug total protein was loaded.

Predicted band size: 35 kDa

false

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human SIRT4 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6E7

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-3 µg/mL", "IHCP-species-notes": "<p>Steamed antigen retrieval with citrate buffer pH 6 is recommended.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

製品の詳細

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SIRT4 also known as Sirtuin 4 is a mitochondrial protein with a mass of approximately 36 kDa. It belongs to the sirtuin family of NAD+-dependent deacetylases. Unlike many other sirtuins SIRT4 does not function as a deacetylase; instead it exhibits ADP-ribosyltransferase activity. This activity regulates a range of mitochondrial functions. SIRT4 is expressed in various tissues including the liver pancreas and muscles where it plays different roles based on the tissue type.

Biological function summary

SIRT4 regulates energy metabolism and cellular stress responses. It plays key roles in the regulation of mitochondrial functions during fasting and impacts gluconeogenesis and fatty acid oxidation. SIRT4 inhibits glutamate dehydrogenase (GDH) affecting amino acid metabolism and insulin secretion. By acting in this capacity SIRT4 controls the flow of nutrients and energy homeostasis exhibiting its critical role in metabolic regulation.

Pathways

SIRT4 integrates into a broader metabolic network including the insulin signaling and AMPK pathways. It interacts with proteins like GDH and AMPK influencing mitochondrial energy output and stress response. This relationship places SIRT4 in an important position for maintaining energy balance and responding to cellular metabolic demands.

SIRT4 links to metabolic conditions such as diabetes and metabolic syndrome. Dysregulation of SIRT4 activity can lead to impaired insulin secretion and glucose intolerance. Furthermore SIRT4's interaction with other sirtuins particularly SIRT1 suggests a functional interplay in protecting against metabolic stress and possibly influencing the development of these metabolic disorders.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Acts as a NAD-dependent protein lipoamidase, biotinylase, deacetylase and ADP-ribosyl transferase (PubMed : 16959573, PubMed : 17715127, PubMed : 24052263, PubMed : 25525879). Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications (PubMed : 24052263, PubMed : 25525879). Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed : 25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity (PubMed : 16959573, PubMed : 17715127). Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1 : expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed : 16959573, PubMed : 17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation (PubMed : 23663782). Acts as a tumor suppressor (PubMed : 23562301, PubMed : 23663782). Also acts as a NAD-dependent protein deacetylase : mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Does not seem to deacetylate PC (PubMed : 23438705). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation (PubMed : 24043310). Impairs SIRT1-PPARA interaction probably through the regulation of NAD(+) levels (PubMed : 24043310). Down-regulates insulin secretion (PubMed : 17715127).

See full target information SIRT4

style

left-column

文献 (19)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed36078054

2022

Autophagy in Rat Müller Glial Cells Is Modulated by the Sirtuin 4/AMPK/mTOR Pathway and Induces Apoptosis under Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqi Qin,Zhi Xie,Ting Cao,Zhiruo Wang,Xiaoyu Zhang,Feifei Wang,Wei Wei,Ming Jin,Jingyuan Ma,Ling Zeng,Yanan Wang,Shaonan Pei,Xu Zhang

Frontiers in oncology 12:872444 PubMed35707358

2022

SIRT4-Catalyzed Deacetylation of Axin1 Modulates the Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Wang,Jicheng Yue,Mingzhe Xiao,Xiaomei Lu,Yuen Eugene Chin

Frontiers in neuroscience 16:840443 PubMed35185463

2022

SIRT4 Is Highly Expressed in Retinal Müller Glial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Piaopiao Hu,Mengqi Qin,Guiping Chen,Feifei Wang,Shengrui Yao,Ming Jin,Zhi Xie,Xu Zhang

Molecular medicine reports 24: PubMed34414449

2021

Circular RNA OMA1 regulates the progression of breast cancer via modulation of the miR‑1276/SIRT4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingli Xu,Ke Xu,Lijun Xiang,Jiamei Yan

Theranostics 10:9458-9476 PubMed32863939

2020

Loss of SIRT4 promotes the self-renewal of Breast Cancer Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lutao Du,Xiaoyan Liu,Yidan Ren,Juan Li,Peilong Li,Qinlian Jiao,Peng Meng,Fang Wang,Yuli Wang,Yun-Shan Wang,Chuanxin Wang

Molecular cell 75:823-834.e5 PubMed31302001

2019

SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Tianshi Wang,Ying Cao,Quan Zheng,Jun Tu,Wei Zhou,Jianli He,Jie Zhong,Yalan Chen,Jiqiu Wang,Rong Cai,Yong Zuo,Bo Wei,Qiuju Fan,Jie Yang,Yicheng Wu,Jing Yi,Dali Li,Mingyao Liu,Chuangui Wang,Aiwu Zhou,Yu Li,Xuefeng Wu,Wen Yang,Y Eugene Chin,Guoqiang Chen,Jinke Cheng

International journal of molecular medicine 44:157-171 PubMed31017270

2019

Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Fan,Kexiang Yan,Qinqin Meng,Rui Sun,Xinrong Yang,Dingfen Yuan,Fulun Li,Hui Deng

Bioscience reports 38: PubMed30442871

2018

Study of expression analysis of and the coordinate regulation of bovine adipocyte differentiation by and its transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Jieyun Hong,Shijun Li,Xiaoyu Wang,Chugang Mei,Linsen Zan

Neuroscience 393:196-205 PubMed30315879

2018

The Stress c-Jun N-terminal Kinase Signaling Pathway Activation Correlates with Synaptic Pathology and Presents A Sex Bias in P301L Mouse Model of Tauopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Buccarello,Clara Alice Musi,Arianna Turati,Tiziana Borsello

Oncology letters 16:1675-1681 PubMed30008852

2018

Knockout of SIRT4 decreases chemosensitivity to 5-FU in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Zhu,Guangyu Wang,Xiaobo Li,Tianzhen Wang,Mingjiao Weng,Yanqiao Zhang

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIRT4 抗体

Anti-SIRT4 antibody

Western blot - Anti-SIRT4 antibody (AB10140)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (19)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise