Anti-SIRT2 抗体 (ab19388)
Key features and details
- Rabbit polyclonal to SIRT2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SIRT2 antibody
SIRT2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SIRT2 -
由来種
Rabbit -
特異性
SIRT2 antibody (ab19388) detects SIRT2 and shows no cross-reactivity to other SIRT isoforms. -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human SIRT2 aa 377-389.
Sequence:DEARTTEREKPQ
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ポジティブ・コントロール
- ICC/IF: SAOS2 transfected cell lysates WB: HeLa and MCF7 whole cell lysate; Mouse brain and liver tissue lysates IHC-P: Human heart tissue
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特記事項
The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide -
Concentration information loading...
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精製度
Whole antiserum -
一次抗体 備考
The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19388の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100.
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WB |
1/250. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
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ICC/IF |
1/400.
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特記事項 |
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IHC-P
1/100. |
WB
1/250. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa). |
ICC/IF
1/400. |
ターゲット情報
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機能
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
組織特異性
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
配列類似性
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
発生段階
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
翻訳後修飾
Phosphorylated at the G2/M transition of the cell cycle. -
細胞内局在
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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参照データベース
- Entrez Gene: 22933 Human
- Entrez Gene: 64383 Mouse
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- SwissProt: Q8VDQ8 Mouse
- Unigene: 466693 Human
- Unigene: 272443 Mouse
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別名
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
see all
画像
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All lanes : Anti-SIRT2 antibody (ab19388) at 1/250 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, HRP conjugate at 1/5000 dilution
Predicted band size: 43 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of SIRT2 showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab19388 diluted in 3% BSA-PBS at a dilution of 1/100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunofluorescence staining of SIRT2 in transfected SAOS2 cells using ab19388.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab19388 は 3 報の論文で使用されています。
- Zhou Q et al. Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3. Bioengineered 13:2017-2027 (2022). PubMed: 34847835
- Lara E et al. Salermide, a Sirtuin inhibitor with a strong cancer-specific proapoptotic effect. Oncogene 28:781-91 (2009). WB ; Human . PubMed: 19060927
- Lovato L et al. Transketolase and CNPase I are specifically recognized by IgG autoantibodies in multiple sclerosis patients. Mol Cell Proteomics : (2008). WB ; Human . PubMed: 18676363