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Anti-SIRP alpha 抗体 [ED9] (ab34086)

Submit a review Q&A (2)

Key features and details

  • Mouse monoclonal [ED9] to SIRP alpha
  • Suitable for: IHC-Fr, IP, WB, Flow Cyt
  • Reacts with: Rat
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-SIRP alpha antibody [ED9]
    SIRP alpha 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [ED9] to SIRP alpha

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Fr, IP, WB, Flow Cytmore details

  • 種交差性

    交差種: Rat

  • 免疫原

    Tissue, cells or virus corresponding to Rat SIRP alpha. Spleen cell homogenate (Rat).
    Database link: P97710

  • 特記事項


    Clone ED9 has been reported to block the interaction of CD172a - CD47. Removal of Sodium Azide is recommended prior to use in functional assays.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab34086の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

  • 組織特異性

    Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.

  • 配列類似性

    Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 翻訳後修飾

    N-glycosylated.
    Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Signal regulatory protein alpha type 1 antibody
      • Bit antibody
      • Brain Ig like molecule with tyrosine based activation motifs antibody
      • Brain Ig-like molecule with tyrosine-based activation motifs antibody
      • Brain immunoglobulin like molecule with tyrosine based activation motifs antibody
      • CD172 antigen like family member A antibody
      • CD172 antigen-like family member A antibody
      • CD172a antibody
      • CD172a antigen antibody
      • Inhibitory receptor SHPS-1 antibody
      • Macrophage fusion receptor antibody
      • MFR antibody
      • MYD 1 antibody
      • Myd 1 antigen antibody
      • MyD-1 antigen antibody
      • p84 antibody
      • Protein tyrosine phosphatase non receptor type substrate 1 antibody
      • PTPNS1 antibody
      • SHP substrate 1 antibody
      • SHPS-1 antibody
      • SHPS1 antibody
      • SHPS1_HUMAN antibody
      • Signal regulatory protein alpha 2 antibody
      • Signal regulatory protein alpha 3 antibody
      • Signal regulatory protein alpha antibody
      • Signal regulatory protein alpha type 2 antibody
      • Signal-regulatory protein alpha-1 antibody
      • Signal-regulatory protein alpha-2 antibody
      • Signal-regulatory protein alpha-3 antibody
      • SIRP antibody
      • Sirp-alpha-1 antibody
      • Sirp-alpha-2 antibody
      • Sirp-alpha-3 antibody
      • SIRPA antibody
      • SIRPalpha antibody
      • SIRPalpha1 antibody
      • SIRPalpha2 antibody
      • SIRPalpha3 antibody
      • Tyrosine phosphatase SHP substrate 1 antibody
      • Tyrosine protein phosphatase non receptor type substrate 1 antibody
      • Tyrosine-protein phosphatase non-receptor type substrate 1 antibody
      see all

    参考文献 (0)

    ab34086 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab34086 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    thank you very much for this kind offer. As we plan to use the TLR4 antibody with rat and human cells we would like to use the ab91532 product as isotype control.

    Read More
    Answer

    I have issued a free of charge replacement for one vial of ab91532. To check the status of the order please contact our Customer Service team and reference this number. Please be aware this product is currently out of stock. Expect delivery around December 21. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

    Read More

    Question

    I need to know what the epitope for anti-SIRP antibodies #s ED9 and OX-41 are. I'm hoping one of them targets to the first 370 amino acids in SIRP. Thanks,

    Read More
    Answer

    Thank you for your enquiry. Regarding ab34011 and ab34086, I am sorry to inform that we do not epitope map our antibodies so unfortunately I　cannot tell you their exact epitopes. However the following reference by　Adams et al shows that these clones bind to different epitopes. Adams S, van der Laan LJ, Vernon-Wilson E, Renardel de Lavalette C, Dopp　EA, Dijkstra CD, Simmons DL, van den Berg TK. Signal-regulatory protein is selectively expressed by myeloid and　neuronal cells.　J Immunol. 1998 Aug 15;161(4):1853-9. PMID: 9712053 I hope the reference above will be able to help. Should you require any further assistance, please do not hesitate to contact me.

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