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Anti-SIN1 抗体 (ab71152)

Submit a review Q&A (2)References (8)
Western blot - Anti-SIN1 antibody (ab71152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIN1 antibody (ab71152)
  • Immunoprecipitation - Anti-SIN1 antibody (ab71152)

Key features and details

  • Rabbit polyclonal to SIN1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SIN1 antibody
    SIN1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to SIN1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat, Sheep, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus

  • 免疫原

    A synthetic peptide corresponding to a region between residue 470 and the C-terminus (residue 522) of human SIN1 (NP_001006618.1).

  • ポジティブ･コントロール

    • Whole cell lysate from Hela cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab71152の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000 - 1/10000. Detects a band of approximately 30, 50, 65 kDa (predicted molecular weight: 59 kDa).
IP
Use at 2-5 µg/mg of lysate.
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/2000 - 1/10000. Detects a band of approximately 30, 50, 65 kDa (predicted molecular weight: 59 kDa).
IP
Use at 2-5 µg/mg of lysate.
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.

  • 組織特異性

    Ubiquitously expressed, with highest levels in heart and skeletal muscle.

  • 配列類似性

    Belongs to the SIN1 family.

  • 細胞内局在

    Cell membrane. Cytoplasmic vesicle. Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • MAPKAP 1 antibody
    • MAPKAP1 antibody
    • MEKK2 interacting protein 1 antibody
    • MGC2745 antibody
    • MIP 1 antibody
    • MIP1 antibody
    • Mitogen activated protein kinase associated protein 1 antibody
    • Mitogen-activated protein kinase 2-associated protein 1 antibody
    • mSIN1 antibody
    • OTTHUMP00000064207 antibody
    • Ras inhibitor MGC2745 antibody
    • SAPK interacting protein 1 antibody
    • SAPK-interacting protein 1 antibody
    • SIN 1 antibody
    • SIN1_HUMAN antibody
    • SIN1b antibody
    • SIN1g antibody
    • Stress activated map kinase interacting protein 1 antibody
    • Stress activated protein kinase interacting 1 antibody
    • Stress-activated map kinase-interacting protein 1 antibody
    • Target of rapamycin complex 2 subunit MAPKAP1 antibody
    • TORC2 subunit MAPKAP1 antibody
    see all

画像

  • Western blot - Anti-SIN1 antibody (ab71152)
    Western blot - Anti-SIN1 antibody (ab71152)
    All lanes : Anti-SIN1 antibody (ab71152) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg

    Predicted band size: 59 kDa
    Observed band size: 60,65 kDa why is the actual band size different from the predicted?
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIN1 antibody (ab71152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIN1 antibody (ab71152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SIN1 with ab71152 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-SIN1 antibody (ab71152)
    Immunoprecipitation - Anti-SIN1 antibody (ab71152)
    Immunoprecipitation/ Western Blot of SIN1
    Lane 1: ab71152 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG IP.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Subsequent WB detection was performed with 1 µg/ml ab71152.
    Chemiluminescence with an exposure time of 30 seconds.

参考文献 (8)

ab71152 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab71152 は 8 報の論文で使用されています。

  • Li ML  et al. The RNA-binding protein AUF1 facilitates Akt phosphorylation at the membrane. J Biol Chem 298:102437 (2022). PubMed: 36041631
  • Zhao Z  et al. Aurora kinase a promotes the progression of papillary thyroid carcinoma by activating the mTORC2-AKT signalling pathway. Cell Biosci 12:195 (2022). PubMed: 36471438
  • Baffi TR  et al. mTORC2 controls the activity of PKC and Akt by phosphorylating a conserved TOR interaction motif. Sci Signal 14:N/A (2021). PubMed: 33850054
  • Zhou H  et al. Inhibitory effect of melatonin on necroptosis via repressing the Ripk3-PGAM5-CypD-mPTP pathway attenuates cardiac microvascular ischemia-reperfusion injury. J Pineal Res N/A:e12503 (2018). PubMed: 29770487
  • Zhou H  et al. NR4A1 aggravates the cardiac microvascular ischemia reperfusion injury through suppressing FUNDC1-mediated mitophagy and promoting Mff-required mitochondrial fission by CK2a. Basic Res Cardiol 113:23 (2018). PubMed: 29744594
  • Drägert K  et al. Basal mTORC2 activity and expression of its components display diurnal variation in mouse perivascular adipose tissue. Biochem Biophys Res Commun 473:317-322 (2016). PubMed: 27016480
  • González-Barriga A  et al. Design and analysis of effects of triplet repeat oligonucleotides in cell models for myotonic dystrophy. Mol Ther Nucleic Acids 2:e81 (2013). WB ; Mouse, Human . PubMed: 23511335
  • Joha S  et al. GILZ inhibits the mTORC2/AKT pathway in BCR-ABL(+) cells. Oncogene : (2011). PubMed: 21804606

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

r
I'd be happy to test then Sin1 Ab in western blot as soon as possible,
whatever the species made in.
And i am very interested in a Rictor antibody. So if you can check if
your others antibodies for Rictor, it will be very nice.
Thank you again,

Read More
Answer

rply. Please find here below the discount code for testing the Anti-SIN1 antibody (ab71152) on Zebra fish.

I have checked also for our other antibodies against Rictor.

We do have 8 antibodies tested in Western blot against Rictor, please see below for comments on each of them. I would recommend to test the https://www.abcam.com/RICTOR-antibody-ab105469.htmlas it is a polyclonal antibody against a short sequence, which is conserved to 93%. Please have however a look and let me know. I will then send you a discount code as well for the selected antibody.Please let me know if you have any questions.


I am very pleased to hear you would like to accept our offer and test ab71152 in Zebra fish. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forZebra fishand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question

Madam, Sir,

I take contact with you because I am interesting in testing two of your antibodies for TorC2 pathway studies.
I work with zebrafish (Danio rerio) model and I'd like to test immunoblots with :
Anti-RICTOR antibody (ab70373) (eventually if WB, test IP)
Anti-SIN1 antibody (ab71152)
Is it possible to have 100% testing discount code ?

Best Regards,

Read More
Answer

Thank you for your inquiry and your interest in our antibodies!

I have contacted the laboratory to find out whether the immunogen sequence is similar to the Danio rerio sequence. The immunogen sequence is proprietary information, however the laboratory confirmed the following:

Sin1: At the site recognized by the antibody, human Sin1 ab71152 and the Zebrafish sequence (XP_003198622.1) are sufficiently similar to expect a probability of about 50% that the antibody ab71152 will bind Sin1 from Zebrafish.

Rictor: At the site recognized by the antibody, human Rictor ab70373 and the Zebrafish sequence (XP_001921907.1) are sufficiently divergent to expect the antibody ab70373 will not bind Rictor from Zebrafish.

I am very happy therefore to provide you with a discount code for the ab71152. Unfortunately I do not think that it would be the ab70373 is a good choice to test. I will be happy to look for possibility of crossreaction to Zebrafish of any of our other antibodies against rictor. Can you confirm please that you need the antibody for Western blot? Does it need to be a rabbit antibody?

We can do discount codes for different antibodies. However one discount code is always linked to test the antibody we have provided. It can however be redeemed against any of our other primary antibodies.

I look forward hearing back from you in to look for another Rictor antibody and to provide also the discount code for ab71152. Please let me know also if you have any questions or concerns.

Read More

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