AB71152

Anti-SIN1 抗体

Name: Anti-SIN1 抗体 (ab71152) | Abcam
Brand: Abcam Limited
SKU: AB71152
Price: 75000 JPY
Availability: InStock

Anti-SIN1 antibody

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(9 Publications)

Rabbit Polyclonal SIN1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human MAPKAP1.

別名を表示する

MIP1, SIN1, MAPKAP1, Target of rapamycin complex 2 subunit MAPKAP1, TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIN1 antibody (AB71152)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIN1 antibody (AB71152)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SIN1 with ab71152 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-SIN1 antibody (AB71152)

Immunoprecipitation/ Western Blot of SIN1
Lane 1 : ab71152 at 3μg/mg whole cell lysate.
Lane 2 : Control IgG IP.

Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Subsequent WB detection was performed with 1 μg/ml ab71152.
Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-SIN1 antibody (ab71152)

Predicted band size: 59 kDa

false

Unknown

Western blot - Anti-SIN1 antibody (AB71152)

All lanes:

Western blot - Anti-SIN1 antibody (ab71152) at 0.1 µg/mL

Lane 1:

Whole cell lysate from Hela cells at 50 µg

Lane 2:

Whole cell lysate from Hela cells at 15 µg

Predicted band size: 59 kDa

Observed band size: 30 kDa,60 kDa,65 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB, IHC-P

applications

免疫原

Synthetic Peptide within Human MAPKAP1. The exact immunogen used to generate this antibody is proprietary information.

Q9BPZ7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SIN1 also known as MAPKAP1 or mTORC2 component is a protein with a mass of approximately 66 kDa. It is an essential part of the mTOR complex 2 (mTORC2) a component critical for controlling cell growth and metabolism. SIN1 is expressed in various tissues indicating its broad role in numerous cellular processes. This expression includes key sites like the liver heart and muscle tissues.

Biological function summary

SIN1 plays an important role in regulating mTORC2 which participates in the phosphorylation of Akt (Protein Kinase B). It forms part of the mTORC2 complex along with mTOR (mechanistic target of rapamycin) and RICTOR (Rapamycin-Insensitive Companion of mTOR). This complex influences cytoskeletal organization cell survival and nutrient sensing important for many cellular functions.

Pathways

SIN1 involvement in mTOR signaling and PI3K-Akt pathways highlights its significance. These pathways are vital for cell growth proliferation and survival. Through mTORC2 it regulates Akt phosphorylation impacting downstream processes including processes controlled by proteins like SGK (serum and glucocorticoid-regulated kinase). SIN1's interactions within these pathways highlight its central role in cell signaling networks.

SIN1 shows links to cancer and diabetes pointing to its influence in disease progression. Alterations in the mTOR signaling pathway involving SIN1 can lead to dysregulated cell growth common in cancerous states. In diabetes the mTOR pathway combined with proteins like insulin receptors affects insulin signaling and glucose homeostasis making SIN1 a target of interest for therapeutic research.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the mechanistic target of rapamycin complex 2 (mTORC2), which transduces signals from growth factors to pathways involved in proliferation, cytoskeletal organization, lipogenesis and anabolic output (PubMed : 15467718, PubMed : 16919458, PubMed : 16962653, PubMed : 17043309, PubMed : 21806543, PubMed : 28264193, PubMed : 28968999, PubMed : 30837283, PubMed : 35926713). In response to growth factors, mTORC2 phosphorylates and activates AGC protein kinase family members, including AKT (AKT1, AKT2 and AKT3), PKC (PRKCA, PRKCB and PRKCE) and SGK1 (PubMed : 16919458, PubMed : 16962653, PubMed : 21806543, PubMed : 28264193, PubMed : 28968999, PubMed : 30837283, PubMed : 35926713). In contrast to mTORC1, mTORC2 is nutrient-insensitive (PubMed : 16962653). Within the mTORC2 complex, MAPKAP1/SIN1 acts as a substrate adapter which recognizes and binds AGC protein kinase family members for phosphorylation by MTOR (PubMed : 21806543, PubMed : 28264193). mTORC2 plays a critical role in AKT1 activation by mediating phosphorylation of different sites depending on the context, such as 'Thr-450', 'Ser-473', 'Ser-477' or 'Thr-479', facilitating the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDPK1/PDK1 which is a prerequisite for full activation (PubMed : 28264193, PubMed : 35926713). mTORC2 catalyzes the phosphorylation of SGK1 at 'Ser-422' and of PRKCA on 'Ser-657' (PubMed : 30837283, PubMed : 35926713). The mTORC2 complex also phosphorylates various proteins involved in insulin signaling, such as FBXW8 and IGF2BP1 (By similarity). mTORC2 acts upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors (PubMed : 15467718). mTORC2 promotes the serum-induced formation of stress-fibers or F-actin (PubMed : 15467718). MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation (PubMed : 15988011). Inhibits HRAS and KRAS independently of mTORC2 complex (PubMed : 17303383, PubMed : 34380736, PubMed : 35522713). Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription (PubMed : 17054722). Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex (PubMed : 23727834).. Isoform 4. In contrast to isoform 1, isoform 2 and isoform 6, isoform 4 is not a component of the a mTORC2 complex.

See full target information MAPKAP1

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 12:195 PubMed36471438

2022

Aurora kinase a promotes the progression of papillary thyroid carcinoma by activating the mTORC2-AKT signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zewei Zhao,Huijuan Wang,Ning Kang,Zhongyu Wang,Xiukun Hou,Linfei Hu,Shuo Qie,Jianping Guo,Songfeng Wei,Xianhui Ruan,Xiangqian Zheng

The Journal of biological chemistry 298:102437 PubMed36041631

2022

The RNA-binding protein AUF1 facilitates Akt phosphorylation at the membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Ling Li,Aparna Ragupathi,Nikhil Patel,Tatiana Hernandez,Jedrick Magsino,Guy Werlen,Gary Brewer,Estela Jacinto

Science signaling 14: PubMed33850054

2021

mTORC2 controls the activity of PKC and Akt by phosphorylating a conserved TOR interaction motif.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy R Baffi,Gema Lordén,Jacob M Wozniak,Andreas Feichtner,Wayland Yeung,Alexandr P Kornev,Charles C King,Jason C Del Rio,Ameya J Limaye,Julius Bogomolovas,Christine M Gould,Ju Chen,Eileen J Kennedy,Natarajan Kannan,David J Gonzalez,Eduard Stefan,Susan S Taylor,Alexandra C Newton

Journal of pineal research 66:e12542 PubMed30516280

2019

Melatonin attenuates myocardial ischemia-reperfusion injury via improving mitochondrial fusion/mitophagy and activating the AMPK-OPA1 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Yue Wang,Junnan Xu,Feng Tian,Shunying Hu,Yundai Chen,Zhenhong Fu

Journal of pineal research 65:e12503 PubMed29770487

2018

Inhibitory effect of melatonin on necroptosis via repressing the Ripk3-PGAM5-CypD-mPTP pathway attenuates cardiac microvascular ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhou,Dandan Li,Pingjun Zhu,Qiang Ma,Sam Toan,Jin Wang,Shunying Hu,Yundai Chen,Yingmei Zhang

Basic research in cardiology 113:23 PubMed29744594

2018

NR4A1 aggravates the cardiac microvascular ischemia reperfusion injury through suppressing FUNDC1-mediated mitophagy and promoting Mff-required mitochondrial fission by CK2α.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhou,Jin Wang,Pingjun Zhu,Hong Zhu,Sam Toan,Shunying Hu,Jun Ren,Yundai Chen

Biochemical and biophysical research communications 473:317-322 PubMed27016480

2016

Basal mTORC2 activity and expression of its components display diurnal variation in mouse perivascular adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Katja Drägert,Indranil Bhattacharya,Michael N Hall,Rok Humar,Edouard Battegay,Elvira Haas

Molecular therapy. Nucleic acids 2:e81 PubMed23511335

2013

Design and analysis of effects of triplet repeat oligonucleotides in cell models for myotonic dystrophy.

Applications

WB, WB

Species

Human, Mouse

Anchel González-Barriga,Susan Am Mulders,Jeroen van de Giessen,Jeroen D Hooijer,Suzanne Bijl,Ingeborg Dg van Kessel,Josee van Beers,Judith Ct van Deutekom,Jack Am Fransen,Bé Wieringa,Derick G Wansink

Oncogene 31:1419-30 PubMed21804606

2011

GILZ inhibits the mTORC2/AKT pathway in BCR-ABL(+) cells.

Applications

Unspecified application

Species

Unspecified reactive species

S Joha,A-L Nugues,D Hétuin,C Berthon,X Dezitter,V Dauphin,F-X Mahon,C Roche-Lestienne,C Preudhomme,B Quesnel,T Idziorek

View all publications

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Abcam product promise

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Anti-SIN1 抗体

Anti-SIN1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIN1 antibody (AB71152)

Immunoprecipitation - Anti-SIN1 antibody (AB71152)

Western blot - Anti-SIN1 antibody (AB71152)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (9)

Abcam product promise