AB5639

Anti-SIL1 抗体

Anti-SIL1 antibody

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(6 Publications)

Goat Polyclonal SIL1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SIL1 aa 400 to C-terminus.

別名を表示する

UNQ545/PRO836, SIL1, Nucleotide exchange factor SIL1, BiP-associated protein, BAP

1 Images

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Western blot - Anti-SIL1 antibody (AB5639)

ab5639 staining (1μg/ml) of Human Kidney lysate (RIPA buffer, 30μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ab5639 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-SIL1 antibody (ab5639)

Predicted band size: 52 kDa

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Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human SIL1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H173

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" } } }

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製品の詳細

SIL1 is a resident endoplasmic reticulum (ER) glycoprotein that interacts with the ATPase domain of BIP and enhances nucleotide exchange.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The SIL1 protein known also as nucleotide exchange factor SIL1 or BAP plays a mechanical role as an endoplasmic reticulum (ER)-localized nucleotide exchange factor for BiP a heat shock protein. SIL1 has a molecular mass of approximately 53 kDa. The expression of SIL1 occurs mainly in tissues with high secretory demand such as the pancreas and liver. It exists prominently within the lumen of the ER where it stabilizes protein folding by facilitating nucleotide exchange on BiP.

Biological function summary

SIL1 operates as a critical component for ER homeostasis and proteostasis. It assists the chaperone function of BiP by promoting ADP/ATP exchange which is an essential step in the protein folding cycle. SIL1 forms part of a complex network of proteins within the ER that regulate protein biosynthesis folding and degradation. Its interactive network ensures efficient ER functioning affecting the maturation and stability of secretory and membrane proteins.

Pathways

SIL1 interacts within both the unfolded protein response (UPR) and ER-associated degradation (ERAD) pathways. In the UPR SIL1's role in BiP activity ensures proper folding of nascent polypeptides reducing ER stress. In ERAD SIL1's actions facilitate misfolded protein processing and clearance. SIL1 is closely related to other chaperones and co-chaperones like GRP78 with whom it collaboratively maintains ER function under stress conditions ensuring cellular homeostasis.

SIL1 mutations associate with Marinesco-Sjögren syndrome a rare autosomal recessive disorder. The syndrome results from disrupted protein homeostasis in the ER leading to multisystem clinical symptoms such as muscle weakness and cataracts. SIL1 also connects to neurodegenerative diseases often characterized by ER stress and faulty proteostasis implicating proteins such as BiP whose impairment also results from SIL1 malfunctions. Understanding these connections is essential for identifying potential therapeutic targets for these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.

See full target information SIL1

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neurology 10:562 PubMed31258504

2019

Identification of Cellular Pathogenicity Markers for SIL1 Mutations Linked to Marinesco-Sjögren Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Gatz,Denisa Hathazi,Ute Münchberg,Stephan Buchkremer,Thomas Labisch,Ben Munro,Rita Horvath,Ana Töpf,Joachim Weis,Andreas Roos

OncoTargets and therapy 11:3775-3783 PubMed29997438

2018

SIL1 functions as an oncogene in glioma by AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Xu,Shangchen Xu,Rui Zhang,Tao Xin,Qi Pang

Nature neuroscience 18:227-38 PubMed25559081

2015

Marinesco-Sjögren syndrome protein SIL1 regulates motor neuron subtype-selective ER stress in ALS.

Applications

IHC-P, IHC-FoFr, IHC-FoFr, IHC-P

Species

Human, Human, Mouse, Mouse

Audrey Filézac de L'Etang,Niran Maharjan,Marisa Cordeiro Braña,Céline Ruegsegger,Ruth Rehmann,Anand Goswami,Andreas Roos,Dirk Troost,Bernard L Schneider,Joachim Weis,Smita Saxena

European journal of human genetics : EJHG 16:961-9 PubMed18285827

2008

Novel SIL1 mutations and exclusion of functional candidate genes in Marinesco-Sjögren syndrome.

Applications

ICC/IF

Species

Mouse

Anna-Kaisa Anttonen,Eija Siintola,Lisbeth Tranebjaerg,Nobue K Iwata,Emilia K Bijlsma,Hiroyuki Meguro,Yaeko Ichikawa,Jun Goto,Outi Kopra,Anna-Elina Lehesjoki

Molecular endocrinology (Baltimore, Md.) 21:376-87 PubMed17095577

2006

The orphan receptor tyrosine kinase Ror2 promotes osteoblast differentiation and enhances ex vivo bone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Ramesh A Bhat,Laura M Seestaller-Wehr,Shoichi Fukayama,Annamarie Mangine,Robert A Moran,Barry S Komm,Peter V N Bodine,Julia Billiard

Nature genetics 37:1309-11 PubMed16282978

2005

The gene disrupted in Marinesco-Sjögren syndrome encodes SIL1, an HSPA5 cochaperone.

Applications

IHC-Fr

Species

Human

Anna-Kaisa Anttonen,Ibrahim Mahjneh,Riikka H Hämäläinen,Clotilde Lagier-Tourenne,Outi Kopra,Laura Waris,Mikko Anttonen,Tarja Joensuu,Hannu Kalimo,Anders Paetau,Lisbeth Tranebjaerg,Denys Chaigne,Michel Koenig,Orvar Eeg-Olofsson,Bjarne Udd,Mirja Somer,Hannu Somer,Anna-Elina Lehesjoki

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-SIL1 抗体

Anti-SIL1 antibody

Western blot - Anti-SIL1 antibody (AB5639)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

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