AB228844

Anti-SIL1 抗体

Anti-SIL1 antibody

JP Deleterious:医薬用外劇物

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(1 Publication)

Rabbit Polyclonal SIL1 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SIL1.

別名を表示する

UNQ545/PRO836, SIL1, Nucleotide exchange factor SIL1, BiP-associated protein, BAP

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIL1 antibody (AB228844)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIL1 antibody (AB228844)

Paraffin-embedded MCF7 xenograft stained for SIL1 with ab228844 at a 1/250 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIL1 antibody (AB228844)

Paraffin-embedded rat muscle tissue stained for SIL1 with ab228844 at a 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SIL1 antibody (AB228844)

10% SDS-PAGE

All lanes:

Western blot - Anti-SIL1 antibody (ab228844) at 1/5000 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 52 kDa

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Supplier Data

Western blot - Anti-SIL1 antibody (AB228844)

10% SDS-PAGE

All lanes:

Western blot - Anti-SIL1 antibody (ab228844) at 1/5000 dilution

All lanes:

Rat kidney tissue extract at 50 µg

Predicted band size: 52 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human SIL1. The exact immunogen used to generate this antibody is proprietary information.

Q9H173

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The SIL1 protein known also as nucleotide exchange factor SIL1 or BAP plays a mechanical role as an endoplasmic reticulum (ER)-localized nucleotide exchange factor for BiP a heat shock protein. SIL1 has a molecular mass of approximately 53 kDa. The expression of SIL1 occurs mainly in tissues with high secretory demand such as the pancreas and liver. It exists prominently within the lumen of the ER where it stabilizes protein folding by facilitating nucleotide exchange on BiP.

Biological function summary

SIL1 operates as a critical component for ER homeostasis and proteostasis. It assists the chaperone function of BiP by promoting ADP/ATP exchange which is an essential step in the protein folding cycle. SIL1 forms part of a complex network of proteins within the ER that regulate protein biosynthesis folding and degradation. Its interactive network ensures efficient ER functioning affecting the maturation and stability of secretory and membrane proteins.

Pathways

SIL1 interacts within both the unfolded protein response (UPR) and ER-associated degradation (ERAD) pathways. In the UPR SIL1's role in BiP activity ensures proper folding of nascent polypeptides reducing ER stress. In ERAD SIL1's actions facilitate misfolded protein processing and clearance. SIL1 is closely related to other chaperones and co-chaperones like GRP78 with whom it collaboratively maintains ER function under stress conditions ensuring cellular homeostasis.

SIL1 mutations associate with Marinesco-Sjögren syndrome a rare autosomal recessive disorder. The syndrome results from disrupted protein homeostasis in the ER leading to multisystem clinical symptoms such as muscle weakness and cataracts. SIL1 also connects to neurodegenerative diseases often characterized by ER stress and faulty proteostasis implicating proteins such as BiP whose impairment also results from SIL1 malfunctions. Understanding these connections is essential for identifying potential therapeutic targets for these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

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ターゲットの情報

Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.

See full target information SIL1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 23:449-463 PubMed31673844

2019

LBX2-AS1/miR-219a-2-3p/FUS/LBX2 positive feedback loop contributes to the proliferation of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yang,Xinhua Dong,Minglong Pu,Hongwei Yang,Weilong Chang,Feihong Ji,Tao Liu,Chongqing Wei,Xiefu Zhang,Xinguang Qiu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SIL1 抗体

Anti-SIL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIL1 antibody (AB228844)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIL1 antibody (AB228844)

Western blot - Anti-SIL1 antibody (AB228844)

Western blot - Anti-SIL1 antibody (AB228844)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Abcam product promise