Anti-Sialyl Tn 抗体 [STn 219] (ab115957)
![Flow Cytometry - Anti-Sialyl Tn antibody [STn 219] (ab115957)](https://www.abcam.co.jp/ps/products/115/ab115957/Images/ab115957-128123-anti-sialyl-tn-antibody-stn-219-flow-cytometry.jpg "Flow Cytometry - Anti-Sialyl Tn antibody [STn 219] (ab115957)")
Key features and details
- Mouse monoclonal [STn 219] to Sialyl Tn
- Suitable for: Flow Cyt
- Reacts with: Sheep, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Sialyl Tn antibody [STn 219] -
製品の詳細
Mouse monoclonal [STn 219] to Sialyl Tn -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Sheep, Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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エピトープ
NeuAc a GalNacOSer/Thr. -
ポジティブ･コントロール
- Human gastrointestinal tumor, prostate and ovary carcinoma tissues.
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特記事項
This product was changed from ascites to tissue culture supernatant on 15th June 2017. The following lots are from ascites and still in stock as of 15th June 2017 (GR271829-1, GR293422-1, GR247695-1, GR293422-2, GR293422-1 ). Lot numbers higher than GR293422-2 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
STn 219 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115957の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
Sialyl-Tn is a carbohydrate antigen overexpressed in several epithelial cancers including breast cancer, and usually associated with poor prognosis. Sialyl-Tn is synthesized by a CMP-Neu5Ac: GalNAc alpha2,6-sialyltransferase: ST6GalNAc I, which catalyzes the transfer of a sialic acid residue in alpha2,6-linkage to the GalNAcalpha1-O-Ser/Thr structure. The resulting disaccharide (Neu5Acalpha2-6GalNAcalpha1-O-Ser/Thr) cannot be further elongated and sialyl-Tn expression results therefore in a shortening of the O-glycan chains -
別名
- CD175s antibody
- Sialosyl Tn antibody
- sTn antibody
画像
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Flow Cytometry - Anti-Sialyl Tn antibody [STn 219] (ab115957)Overlay histogram showing MCF7 cells stained with ab115957 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115957, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab115957 は 8 報の論文で使用されています。
- Lenza MP et al. Structural insights into Siglec-15 reveal glycosylation dependency for its interaction with T cells through integrin CD11b. Nat Commun 14:3496 (2023). PubMed: 37311743
- Wu J et al. Screening and Identification of a Novel Anti-Siglec-15 Human Antibody 3F1 and Relevant Antitumor Activity. Mol Pharmacol 102:161-171 (2022). PubMed: 35764384
- Xu F et al. Demethylation of the Cosmc Promoter Alleviates the Progression of Breast Cancer Through Downregulation of the Tn and Sialyl-Tn Antigens. Cancer Manag Res 12:1017-1027 (2020). PubMed: 32104083
- Huang L et al. Elevated miR-124-3p in the aging colon disrupts mucus barrier and increases susceptibility to colitis by targeting T-synthase. Aging Cell 19:e13252 (2020). PubMed: 33040455
- Kvorjak M et al. Cross-talk between Colon Cells and Macrophages Increases ST6GALNAC1 and MUC1-sTn Expression in Ulcerative Colitis and Colitis-Associated Colon Cancer. Cancer Immunol Res 8:167-178 (2020). PubMed: 31831633
- Wolters-Eisfeld G et al. Loss of complex O-glycosylation impairs exocrine pancreatic function and induces MODY8-like diabetes in mice. Exp Mol Med 50:133 (2018). PubMed: 30305605
- NguyenHoang S et al. High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery. Oncotarget 8:80083-80092 (2017). PubMed: 29108390
- Prendergast JM et al. Novel anti-Sialyl-Tn monoclonal antibodies and antibody-drug conjugates demonstrate tumor specificity and anti-tumor activity. MAbs 9:615-627 (0). PubMed: 28281872