AB115957

Anti-Sialyl Tn 抗体 [STn 219]

Name: Anti-Sialyl Tn 抗体 [STn 219] (ab115957) | Abcam
Brand: Abcam Limited
SKU: AB115957
Price: 60000 JPY
Availability: InStock

Anti-Sialyl Tn antibody [STn 219]

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(12 Publications)

Mouse Monoclonal Sialyl Tn antibody. Suitable for Flow Cyt and reacts with Human, Sheep samples. Cited in 12 publications.

別名を表示する

CD175s, Sialosyl Tn, sTn

1 Images

Flow Cytometry - Anti-Sialyl Tn antibody [STn 219] (AB115957)

Flow Cyt

Unknown

Flow Cytometry - Anti-Sialyl Tn antibody [STn 219] (AB115957)

Overlay histogram showing MCF7 cells stained with ab115957 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115957, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

STn 219

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

交差種

Sheep, Human

アプリケーション

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

エピトープ

NeuAc a GalNacOSer/Thr.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Sheep": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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製品の詳細

This product was changed from ascites to tissue culture supernatant on 15th June 2017. The following lots are from ascites and still in stock as of 15th June 2017 (GR271829-1, GR293422-1, GR247695-1, GR293422-2, GR293422-1 ). Lot numbers higher than GR293422-2 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Sialyl Tn

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4912 PubMed40425538

2025

NXPE1 alters the sialoglycome by acetylating sialic acids in the human colon.

Applications

Unspecified application

Species

Unspecified reactive species

Bum Seok Lee,Ashley Cook,Surojit Sur,Laura Dobbyn,Maria Popoli,Sana Khalili,Shibin Zhou,Chetan Bettegowda,Nickolas Papadopoulos,Kathy Gabrielson,Phillip Buckhaults,Bert Vogelstein,Kenneth W Kinzler,Nicolas Wyhs

Nature communications 14:3496 PubMed37311743

2023

Structural insights into Siglec-15 reveal glycosylation dependency for its interaction with T cells through integrin CD11b.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Pia Lenza,Leire Egia-Mendikute,Asier Antoñana-Vildosola,Cátia O Soares,Helena Coelho,Francisco Corzana,Alexandre Bosch,Prodhi Manisha,Jon Imanol Quintana,Iker Oyenarte,Luca Unione,María Jesús Moure,Mikel Azkargorta,Unai Atxabal,Klaudia Sobczak,Felix Elortza,James D Sutherland,Rosa Barrio,Filipa Marcelo,Jesús Jiménez-Barbero,Asis Palazon,June Ereño-Orbea

The EMBO journal 42:e111869 PubMed36245281

2022

The disulfide catalyst QSOX1 maintains the colon mucosal barrier by regulating Golgi glycosyltransferases.

Applications

Unspecified application

Species

Unspecified reactive species

Tal Ilani,Nava Reznik,Noa Yeshaya,Tal Feldman,Patrick Vilela,Zipora Lansky,Gabriel Javitt,Michal Shemesh,Ori Brenner,Yoav Elkis,Neta Varsano,Ana M Jaramillo,Christopher M Evans,Deborah Fass

Frontiers in molecular biosciences 9:985648 PubMed36172045

2022

Curcumin-mediated transcriptional regulation of human -acetylgalactosamine-α2,6-sialyltransferase which synthesizes sialyl-Tn antigen in HCT116 human colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

So-Young An,Kyoung-Sook Kim,Jong-Hyun Cho,Hee-Do Kim,Cheorl-Ho Kim,Young-Choon Lee

Molecular pharmacology 102:161-171 PubMed35764384

2022

Screening and Identification of a Novel Anti-Siglec-15 Human Antibody 3F1 and Relevant Antitumor Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaguo Wu,Jingyi Peng,Yangyihua Zhou,Ran Zhang,Zhihong Wang,Naijing Hu,Dingmu Zhang,Guiqi Quan,Yuanyu Wu,Jiannan Feng,Beifen Shen,Jian Zhao,Yan Zhang,Kaiming Yang,Longlong Luo

Aging cell 19:e13252 PubMed33040455

2020

Elevated miR-124-3p in the aging colon disrupts mucus barrier and increases susceptibility to colitis by targeting T-synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Li Huang,Ting-Yi Sun,Liang-Jun Hu,Shi-Long Hu,Hai-Mei Sun,Fu-Qian Zhao,Bo Wu,Shu Yang,Feng-Qing Ji,De-Shan Zhou

Cancer management and research 12:1017-1027 PubMed32104083

2020

Demethylation of the Cosmc Promoter Alleviates the Progression of Breast Cancer Through Downregulation of the Tn and Sialyl-Tn Antigens.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Xu,Dong Wang,JianXiu Cui,Jie Li,Hongchuan Jiang

Cancer immunology research 8:167-178 PubMed31831633

2019

Cross-talk between Colon Cells and Macrophages Increases ST6GALNAC1 and MUC1-sTn Expression in Ulcerative Colitis and Colitis-Associated Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Kvorjak,Yasmine Ahmed,Michelle L Miller,Raahul Sriram,Claudia Coronnello,Jana G Hashash,Douglas J Hartman,Cheryl A Telmer,Natasa Miskov-Zivanov,Olivera J Finn,Sandra Cascio

Experimental & molecular medicine 50:1-13 PubMed30305605

2018

Loss of complex O-glycosylation impairs exocrine pancreatic function and induces MODY8-like diabetes in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Gerrit Wolters-Eisfeld,Baris Mercanoglu,Bianca T Hofmann,Thomas Wolpers,Claudia Schnabel,Sönke Harder,Pascal Steffen,Kai Bachmann,Babett Steglich,Jörg Schrader,Nicola Gagliani,Hartmut Schlüter,Cenap Güngör,Jakob R Izbicki,Christoph Wagener,Maximilian Bockhorn

mAbs 9:615-627 PubMed28281872

2017

Novel anti-Sialyl-Tn monoclonal antibodies and antibody-drug conjugates demonstrate tumor specificity and anti-tumor activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jillian M Prendergast,Ana Paula Galvao da Silva,David A Eavarone,Darius Ghaderi,Mai Zhang,Dane Brady,Joan Wicks,Julie DeSander,Jeff Behrens,Bo R Rueda

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sialyl Tn 抗体 [STn 219]

Anti-Sialyl Tn antibody [STn 219]

Flow Cytometry - Anti-Sialyl Tn antibody [STn 219] (AB115957)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (12)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise