Anti-Sialoadhesin/CD169 抗体 [SP213] - BSA and Azide free (ab245735)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human lung tissue.

Panel A: Merged staining of anti-Podoplanin / gp36 (magenta; Opal™690), anti-Surfactant protein D/SP-D (green; Opal™520) and anti-Sialoadhesin/CD169 (red; Opal™570) on human lung.
Panel B: Anti-Podoplanin / gp36 stained on alveolar type I cells.
Panel C: Anti-Surfactant protein D/SP-D stained on alveolar type II cells.
Panel D: Anti-Sialoadhesin/CD169 stained on macrophages.

The section was incubated in three rounds of staining: in the order of ab236529 and ab234257 for 30 mins, then ab245735 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Sialoadhesin/CD169 with ab227672 at 1/100 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227672)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Sialoadhesin/CD169 with ab227672 at 1/100 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227672)

Intracellular Flow Cytometry analysis of Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells labeling Sialoadhesin/CD169 with purified ab227672 at 1/20 dilution (6µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue. 

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227672). 

 

 

Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227672).

Formalin-fixed, paraffin-embedded human lung tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227672).

Formalin-fixed, paraffin-embedded human colon tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227672).

Formalin-fixed, paraffin-embedded human bone marrow tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227672).

Formalin-fixed, paraffin-embedded human liver tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227672).