Anti-SIAE 抗体 (ab69300)
Key features and details
- Mouse polyclonal to SIAE
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SIAE antibody -
製品の詳細
Mouse polyclonal to SIAE -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human SIAE. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- Human colon tissue lysate. SIAE transfected 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69300の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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| 特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
ターゲット情報
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関連性
Sialic acids are acidic 9-carbon sugars typically found at the non-reducing end of sugar chains. They are frequently modified by 9-O-acetylation and this modification is removed by sialic acid acetylesterases. SIAE catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. SIAE appears to encode both lysosomal and cytosolic sialic acid acetylesterase isoforms (LSE and CSE, respectively). It is widely expressed with high expression in the testis, prostate, and colon. There are three named isoforms. -
細胞内局在
Lysosome -
参照データベース
- Entrez Gene: 54414 Human
- Omim: 610079 Human
- SwissProt: Q9HAT2 Human
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別名
- CSE C antibody
- CSEC antibody
- Cytosolic sialic acid 9 O acetylesterase homolog antibody
see all
画像
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Western blot - Anti-SIAE antibody (ab69300)Anti-SIAE antibody (ab69300) at 1/500 dilution + human colon tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
Additional bands at: 21 kDa, 26 kDa, 32 kDa, 35 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-SIAE antibody (ab69300)All lanes : Anti-SIAE antibody (ab69300) at 1/500 dilution
Lane 1 : SIAE transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
データシートおよび資料
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Datasheet download
参考文献 (0)
ab69300 は論文での使用が確認できていません。
