Anti-SHPRH 抗体 (ab80129)
Key features and details
- Rabbit polyclonal to SHPRH
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SHPRH antibody -
製品の詳細
Rabbit polyclonal to SHPRH -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human SHPRH aa 600-700 conjugated to keyhole limpet haemocyanin.
(Peptide available asab89391) -
ポジティブ･コントロール
- This antibody gave a positive signal in SHSY-5Y whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80129の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa).
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa). |
ターゲット情報
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機能
E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'. -
組織特異性
Broadly expressed. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the SNF2/RAD54 helicase family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
ドメイン
The RING finger mediates E3 ubiquitin ligase activity. - Information by UniProt
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参照データベース
- Entrez Gene: 257218 Human
- Omim: 608048 Human
- SwissProt: Q149N8 Human
- Unigene: 723297 Human
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別名
- bA545I5.2 antibody
- E3 ubiquitin-protein ligase SHPRH antibody
- FLJ27258 antibody
see all
画像
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Western blot - Anti-SHPRH antibody (ab80129)This image is courtesy of an anonymous AbreviewAnti-SHPRH antibody (ab80129) at 1/1000 dilution + HEK293 whole cell lysate at 50 µg
Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 193 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-SHPRH antibody (ab80129)ICC/IF image of ab80129 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) SHSY5Y cells at 5µg/ml. -
Western blot - Anti-SHPRH antibody (ab80129)Anti-SHPRH antibody (ab80129) at 1 µg/ml + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 193 kDa
Observed band size: 104,193 kDa why is the actual band size different from the predicted?
Additional bands at: 172 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3,4 and 5) of E3 ubiquitin-protein ligase SHPRH. The predicted molecular weights of isoforms (1,2,3,4 and 5) are 193 kDa, 104kDa, 17 kDa, 190 kDa and 122 kDa respectively.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab80129 は 4 報の論文で使用されています。
- Moore CE et al. RFWD3 promotes ZRANB3 recruitment to regulate the remodeling of stalled replication forks. J Cell Biol 222:N/A (2023). PubMed: 37036693
- Gallina I et al. The ubiquitin ligase RFWD3 is required for translesion DNA synthesis. Mol Cell 81:442-458.e9 (2021). PubMed: 33321094
- Waraky A et al. Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after DNA damage. J Biol Chem 292:18227-18239 (2017). PubMed: 28924044
- Qu Y et al. Axitinib blocks Wnt/ß-catenin signaling and directs asymmetric cell division in cancer. Proc Natl Acad Sci U S A 113:9339-44 (2016). WB . PubMed: 27482107