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AB9214

Anti-SHP2 抗体

Anti-SHP2 antibody

4

(1 Review)

|

(5 Publications)

Goat Polyclonal SHP2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PTPN11 aa 550 to C-terminus.

別名を表示する

PTP2C, SHPTP2, PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, SHP-2, Shp2

4 Images
Flow Cytometry (Intracellular) - Anti-SHP2 antibody (AB9214)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SHP2 antibody (AB9214)

Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton-permeabilized A431 cells (blue) staining SHP2 with ab9214 at 10μg/ml, followed by Alexa Fluor 488 secondary antibody at 1ug/ml. Primary incubation carried out for one hour.

IgG control : Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody (black).

Immunocytochemistry/ Immunofluorescence - Anti-SHP2 antibody (AB9214)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHP2 antibody (AB9214)

Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% triton-permeabilized HeLa cells staining SHP2 antibody with ab9214 at 10μg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. DAPI was used as a nuclear counterstain.

Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody at 2ug/ml

Western blot - Anti-SHP2 antibody (AB9214)
  • WB

Unknown

Western blot - Anti-SHP2 antibody (AB9214)

ab9214 staining (2μg/ml) of Human Muscle lysate (RIPA buffer, 35μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab9214 staining (2µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-SHP2 antibody (ab9214)

Predicted band size: 68 kDa

false

Western blot - Anti-SHP2 antibody (AB9214)
  • WB

Unknown

Western blot - Anti-SHP2 antibody (AB9214)

All lanes:

Western blot - Anti-SHP2 antibody (ab9214) at 2 µg/mL

All lanes:

Human muscle lysate at 35 µg

Predicted band size: 68 kDa

false

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt (Intra), ICC/IF, WB

applications

免疫原

Synthetic Peptide within Human PTPN11 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q06124

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.
Biological function summary

The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.

Pathways

SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.

SHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed : 10655584, PubMed : 14739280, PubMed : 18559669, PubMed : 18829466, PubMed : 26742426, PubMed : 28074573). Positively regulates MAPK signal transduction pathway (PubMed : 28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed : 28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed : 18559669). Dephosphorylates CDC73 (PubMed : 26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (PubMed : 19275884).
See full target information PTPN11
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

MedComm 3:e120 PubMed35281792

2022

Tyrosine phosphatase SHP2 exacerbates psoriasis-like skin inflammation in mice via ERK5-dependent NETosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Ding,Zijun Ouyang,Chenyang Zhang,Yuyu Zhu,Qiang Xu,Haiyan Sun,Jiao Qu,Yang Sun

Scientific reports 10:2505 PubMed32054954

2020

SLAM family member 8 is expressed in and enhances the growth of anaplastic large cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiko Sugimoto,Tatsuki R Kataoka,Hiroaki Ito,Kyohei Kitamura,Narumi Saito,Masahiro Hirata,Chiyuki Ueshima,Yusuke Takei,Koki Moriyoshi,Yasuyuki Otsuka,Momoko Nishikori,Akifumi Takaori-Kondo,Hironori Haga

Experimental dermatology 27:641-646 PubMed29498772

2018

SLAM family member 8 is involved in oncogenic KIT-mediated signalling in human mastocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiko Sugimoto,Tatsuki R Kataoka,Chiyuki Ueshima,Yusuke Takei,Kyohei Kitamura,Masahiro Hirata,Takashi Nomura,Hironori Haga

Cellular signalling 23:487-96 PubMed21070852

2010

Association of supervillin with KIR2DL1 regulates the inhibitory signaling of natural killer cells.

Applications

WB

Species

Human

Hai-Peng Liu,Ming-Can Yu,Meng-Hui Jiang,Jian-Xia Chen,Da-Peng Yan,Feng Liu,Bao-Xue Ge

Journal of immunology (Baltimore, Md. : 1950) 174:1801-10 PubMed15699106

2005

Modulation of TNF-alpha gene expression by IFN-gamma and pamidronate in murine macrophages: regulation by STAT1-dependent pathways.

Applications

WB

Species

Mouse

Kae Takagi,Masatoshi Takagi,Siva Kanangat,Kenneth J Warrington,Hidenobu Shigemitsu,Arnold E Postlethwaite
View all publications
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