Anti-SHMT2/SHMT 抗体 - C-terminal (ab180786)
Key features and details
- Rabbit polyclonal to SHMT2/SHMT - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SHMT2/SHMT antibody - C-terminal
SHMT2/SHMT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SHMT2/SHMT - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human SHMT2/SHMT aa 265-504 (C terminal).
Database link: P34897 -
ポジティブ･コントロール
- WB: HeLa, OVCAR3 and 22Rv1 cell lysates, mouse liver and kidney tissue lysates; IHC: Human liver cancer tissue, mouse and rat spleen tissues; ICC/ IF: L929 and U-2 OS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180786の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/500 - 1/2000. Predicted molecular weight: 56 kDa.
|
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| ICC/IF | (1) |
1/50 - 1/200.
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| 特記事項 |
|---|
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IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/500 - 1/2000. Predicted molecular weight: 56 kDa. |
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ICC/IF
1/50 - 1/200. |
ターゲット情報
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機能
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA. -
パスウェイ
One-carbon metabolism; tetrahydrofolate interconversion. -
配列類似性
Belongs to the SHMT family. -
細胞内局在
Mitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6472 Human
- Entrez Gene: 108037 Mouse
- Entrez Gene: 299857 Rat
- Omim: 138450 Human
- SwissProt: P34897 Human
- Unigene: 741179 Human
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別名
- Epididymis secretory sperm binding protein Li 51e antibody
- GLY A+ antibody
- GLYA antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)
Paraffin-embedded human liver cancer tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Immunofluorescence staining of L929 cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)All lanes : Anti-SHMT2/SHMT antibody - C-terminal (ab180786) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : OVCAR3 cell lysate
Lane 3 : 22Rv1 cell lysate
Lane 4 : Mouse liver tissue lysate
Lane 5 : Mouse kidney tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 56 kDaBlocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Paraffin-embedded mouse spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Immunofluorescence staining of U-2 OS cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Paraffin-embedded rat spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab180786 は 7 報の論文で使用されています。
- Thompson LL et al. Reduced SKP1 Expression Induces Chromosome Instability through Aberrant Cyclin E1 Protein Turnover. Cancers (Basel) 12:N/A (2020). PubMed: 32106628
- Yang Y et al. RRM2 protects against ferroptosis and is a tumor biomarker for liver cancer. Cancer Cell Int 20:587 (2020). PubMed: 33372599
- Qi C et al. Circ_0072995 Promotes Cell Carcinogenesis via Up-Regulating miR-149-5p-Mediated SHMT2 in Breast Cancer. Cancer Manag Res 12:11169-11181 (2020). PubMed: 33173349
- Xu T et al. MicroRNA-940 inhibits glioma progression by blocking mitochondrial folate metabolism through targeting of MTHFD2. Am J Cancer Res 9:250-269 (2019). PubMed: 30906627
- Sdelci S et al. MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation. Nat Genet 51:990-998 (2019). PubMed: 31133746
- Wu M et al. Overexpression of mitochondrial serine hydroxyl-methyltransferase 2 is associated with poor prognosis and promotes cell proliferation and invasion in gliomas. Onco Targets Ther 10:3781-3788 (2017). PubMed: 28794642
- Yin K Positive correlation between expression level of mitochondrial serine hydroxymethyltransferase and breast cancer grade. Onco Targets Ther 8:1069-74 (2015). IHC-P ; Human . PubMed: 25999742