Anti-SHMT1 抗体 (ab186130)
Key features and details
- Rabbit polyclonal to SHMT1
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SHMT1 antibody
SHMT1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SHMT1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human SHMT1 aa 450 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_004160.3).
Database link: P34896 -
ポジティブ･コントロール
- WB: Jurkat and NIH 3T3 whole cell lysates. IP: Jurkat cell lysate. IHC-P: Human lung cancer and mouse teratoma tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab186130 was affinity purified using an epitope specific to SHMT1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186130の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/500 - 1/2500. Predicted molecular weight: 53 kDa.
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| IHC-P |
1/500 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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IP
Use at 2-10 µg/mg of lysate. |
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WB
1/500 - 1/2500. Predicted molecular weight: 53 kDa. |
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IHC-P
1/500 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Interconversion of serine and glycine. -
パスウェイ
One-carbon metabolism; tetrahydrofolate interconversion. -
配列類似性
Belongs to the SHMT family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 454490 Chimpanzee
- Entrez Gene: 102122367 Cynomolgus monkey
- Entrez Gene: 6470 Human
- Entrez Gene: 20425 Mouse
- Entrez Gene: 100171460 Orangutan
- Omim: 182144 Human
- SwissProt: P34896 Human
- SwissProt: P50431 Mouse
see all -
別名
- CSHMT antibody
- Cytoplasmic serine hydroxymethyltransferase antibody
- cytosolic antibody
see all
画像
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Western blot - Anti-SHMT1 antibody (ab186130)Anti-SHMT1 antibody (ab186130) at 1 µg/ml + Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 3 minutes -
Western blot - Anti-SHMT1 antibody (ab186130)Anti-SHMT1 antibody (ab186130) at 1 µg/ml + NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-SHMT1 antibody (ab186130)Detection of SHMT1 in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186130 at 6 µg per reaction for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT1 antibody (ab186130)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human lung cancer tissue labeling SHMT1 with ab186130 at a 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT1 antibody (ab186130)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded mouse teratoma tissue labeling SHMT1 with ab186130 at a 1/1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab186130 は 7 報の論文で使用されています。
- Wei J et al. Identification of AGXT2, SHMT1, and ACO2 as important biomarkers of acute kidney injury by WGCNA. PLoS One 18:e0281439 (2023). PubMed: 36735737
- Chen Y et al. Use of recombinant microRNAs as antimetabolites to inhibit human non-small cell lung cancer. Acta Pharm Sin B 13:4273-4290 (2023). PubMed: 37799388
- Zhao L et al. mTORC1-c-Myc pathway rewires methionine metabolism for HCC progression through suppressing SIRT4 mediated ADP ribosylation of MAT2A. Cell Biosci 12:183 (2022). PubMed: 36371321
- Zhou X et al. Coordinated pyruvate kinase activity is crucial for metabolic adaptation and cell survival during mitochondrial dysfunction. Hum Mol Genet 30:2012-2026 (2021). PubMed: 34169315
- May JL et al. IDH3a regulates one-carbon metabolism in glioblastoma. Sci Adv 5:eaat0456 (2019). PubMed: 30613765
- Dou C et al. SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production. J Exp Clin Cancer Res 38:70 (2019). PubMed: 30755243
- Sdelci S et al. MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation. Nat Genet 51:990-998 (2019). PubMed: 31133746