Anti-SHC 抗体 [EP332Y]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody [EP332Y] (AB33770)

ab33770 staining SHC in Human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody [EP332Y] (AB33770)

ab33770 staining SHC in Human renal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

• WB

Supplier Data

Western blot - Anti-SHC antibody [EP332Y] (AB33770)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-SHC antibody [EP332Y] (ab33770) at 1/1000 dilution

Lane 1:

MCF-7 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 62 kDa

false

• WB

Lab

Western blot - Anti-SHC antibody [EP332Y] (AB33770)

Western blot : Anti-SHC antibody [EP332Y] ab33770 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 50, 55, 100-140 kDa in Wild-type A549 cell lysates with no signal observed at this size in SHC1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SHC antibody [EP332Y] (ab33770) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

SHC1 knockout A549 at 20 µg

Lane 3:

HT1080 at 20 µg

Lane 4:

Raji at 20 µg

Lane 5:

HL-60 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 100-140 kDa

Observed band size: 55 kDa,50 kDa,63 kDa,37 kDa

false

• WB

Unknown

Western blot - Anti-SHC antibody [EP332Y] (AB33770)

All lanes:

Western blot - Anti-SHC antibody [EP332Y] (ab33770) at 1/2000 dilution

All lanes:

MCF 7 cell lysate

Predicted band size: 62 kDa

Observed band size: 46 kDa,52 kDa,66 kDa

false

• WB

Supplier Data

Western blot - Anti-SHC antibody [EP332Y] (AB33770)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-SHC antibody [EP332Y] (ab33770) at 1/1000 dilution

Lane 1:

C6 Cell lysate at 20 µg

Lane 2:

RAW264.7 Cell lysate at 20 µg

Lane 3:

NIH/3T3 Cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 62 kDa

false