Anti-SH3GL1/Endophilin A2 抗体 [K51/1]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SH3GL1/Endophilin A2 with ab317835 at 1/1000 (1.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on rat cardiac muscle.
The section was incubated with ab317835 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SH3GL1/Endophilin A2 with ab317835 at 1/1000 (1.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining on rat cerebrum.
The section was incubated with ab317835 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

SH3GL1/Endophilin A2 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab317835 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317835 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate
Lane 2 : ab317835 IP in Rat brain tissue lysate
Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab317835 in rat testis tissue lysate

All lanes:

Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835) at 1/30 dilution

All lanes:

Rat brain tissue lysate at 0.35 mg with NFDM/TBST

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 41s

• IP

Supplier Data

Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

SH3GL1/Endophilin A2 was immunoprecipitated from 0.35 mg Rat testis tissue lysate with ab317835 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317835 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat testis tissue lysate
Lane 2 : ab317835 IP in Rat testis tissue lysate
Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab317835 in rat testis tissue lysate

All lanes:

Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835) at 1/30 dilution

All lanes:

Rat testis tissue lysate with NFDM/TBST

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 41s

• WB

Supplier Data

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

Low expression : heart and kidney (PMID : 11518713).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg with NFDM/TBST

Lane 2:

Rat heart tissue lysate at 20 µg with NFDM/TBST

Lane 3:

Rat kidney tissue lysate at 20 µg with NFDM/TBST

Lane 4:

Rat spleen tissue lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 41 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (AB317835)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 2:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 3:

L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg with NFDM/TBST

Lane 4:

AR42J (rat pancreatic tumor epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 5:

H9c2(2-1) (rat cardiomyoblast) whole cell lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 41 kDa,36 kDa

false

Exposure time: 180s