JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB321807

Anti-SH3GL1/Endophilin A2 抗体 [EPR29123-74]

Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74]

  • BOND RX™ Validated
  • 20ul selling size
  • Recombinant
  • RabMAb
  • KO Validated
  • 詳細を見る

Be the first to review this product! Submit a review

|

(0 Publication)

Knockout Tested Rabbit Recombinant Monoclonal SH3G1 antibody. Suitable for Dot, ICC/IF, WB, IHC-P and reacts with Recombinant fragment - Human, Human samples.

別名を表示する

CNSA1, SH3D2B, Endophilin-A2, EEN fusion partner of MLL, Endophilin-2, Extra eleven-nineteen leukemia fusion gene protein, SH3 domain protein 2B, SH3 domain-containing GRB2-like protein 1, EEN

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling SH3GL1/Endophilin A2 with ab321807 at 1/1000 (0.509 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human endometrium (PMID : 9122235).
The section was incubated with ab321807 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SH3GL1/Endophilin A2 with ab321807 at 1/1000 (0.509 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on human skeletal muscle (PMID : 9238017).
The section was incubated with ab321807 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SH3GL1/Endophilin A2 with ab321807 at 1/1000 (0.509 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cerebrum (PMID : 23050879).
The section was incubated with ab321807 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed 0.1% TritonX-100 permeabilized SH3GL1 KO HEK293T (SH3GL1 knockout human embryonic kidney epithelial cell) cells labelling SH3GL1/Endophilin A2 with ab321807 at 1/500 (1.018 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in HEK293T cell line (shown in green) and no staining in SH3GL1 KO HEK293T cell line. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)

Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK293T (human epithelial cell line from embryonic kidney) cell pellet (B) SH3GL1 knockout HEK293T cell pellet tissue labeling SH3GL1/Endophilin A2 with ab321807 at 1/2000 (0.255 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) Wild-type HEK293T cell pellet negative staining on (B) SH3GL1 knockout HEK293T cell pellet.
The section was incubated with ab321807 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling SH3GL1/Endophilin A2 with ab321807 at 1/1000 (0.509 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human spleen (PMID : 9122235).
The section was incubated with ab321807 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)
  • WB

Supplier Data

Western blot - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab321807 was shown to bind specifically to SH3GL1. Target of interest was observed at 36 and 48 kDa in wild-type HEK 293T cell lysates (lane 1) with no signal observed at this size in SH3GL1 knockout cell line (ab259109) (lane 2).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (ab321807) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

SH3GL1 knockout HEK-293T whole cell lysate at 20 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

SH-SY5Y (human neuroblastoma epithelial cell) whole lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 36 kDa,48 kDa,124 kDa

false

Exposure time: 59s

Western blot - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)
  • WB

Supplier Data

Western blot - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Jurkat(PMID : 16990610), skeletal muscle(PMID : 9238017)

lane 1&2 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 and lanes 3-5 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (ab321807) at 1/1000 dilution

Lane 1:

Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 20 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 3:

Human breast tissue lysate at 20 µg

Lane 4:

Human colon tissue lysate at 20 µg

Lane 5:

Human skeletal muscle tissue lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 3 - 5:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 48 kDa,36 kDa

false

Exposure time: 103s

Dot Blot - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)
  • Dot

Supplier Data

Dot Blot - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (AB321807)

Dot blot analysis of SH3GL1/Endophilin A2 using ab321807 at 1 : 1000 (0.509 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 10,000 dilution.

Lane1 : His-tagged human SH3GL1 fragment
Lane2 : His-tagged human SH3GL2 fragment
Lane3 : His-tagged human SH3GL3 fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human SH3GL2 and SH3GL3.

All lanes:

Dot Blot - Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] (ab321807) at 1/1000 dilution

Lane 1:

His-tagged human SH3GL1 fragment

Lane 2:

His-tagged human SH3GL2 fragment

Lane 3:

His-tagged human SH3GL3 fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

false

Exposure time: 180s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-SH3GL1/Endophilin A2 antibody [EPR29123-74] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR29123-74

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, ICC/IF, IHC-P, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
style
left-column

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SH3GL1 also known as Endophilin A2 is a protein that facilitates membrane curvature during endocytosis through its BAR domain. It weighs approximately 42 kDa. Endophilin A2 is widely expressed in various tissues with notable abundance in the brain. It functions by binding to membrane lipids and interacting with other endocytic proteins. This contributes to the formation of vesicles that ferry molecules into cells vital for nutrient uptake and receptor recycling.
Biological function summary

SH3GL1/Endophilin A2 participates in synaptic vesicle endocytosis a process critical for neurotransmission and proper brain function. It works in association with other proteins in a complex such as dynamin to mediate rapid membrane invagination and fission. By assisting in vesicle formation and release at synaptic terminals it plays a role in maintaining synaptic efficacy and neuronal communication.

Pathways

This protein plays a significant role in the clathrin-mediated endocytosis and synaptic vesicle cycle pathways. In these contexts Endophilin A2 interacts with proteins like clathrin and amphiphysin to enable efficient vesicle budding and release. Its activities connect to the recycling of synaptic vesicles essential for the preservation of synaptic transmission and cell signaling.

SH3GL1/Endophilin A2 associates with neurodegenerative diseases like Alzheimer's disease. Dysfunctional endocytosis resulting from its altered expression or activity can impair synaptic function contributing to disease progression. Other disorders involving this protein include certain types of cancer where its interactions with proteins such as c-Cbl may impact cell proliferation and survival influencing tumorigenesis.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).
See full target information SH3GL1
style
left-column
style
left-column
style
right-column

組合せ製品

Primary Antibodies

AB18203

Anti-N Cadherin antibody - Intercellular Junction Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (AB18203)

  • 4
  • 48
Cell Lines & Lysates

AB266260

Human SH3GL1 (EEN) knockout HEK-293T cell line

Advanced Validation

Western blot - Human SH3GL1 (EEN) knockout HEK-293T cell line (AB266260)

  • 0
  • 0
Cell Lines & Lysates

AB259109

Human SH3GL1 (EEN) knockout HEK-293T cell lysate

Western blot - Human SH3GL1 (EEN) knockout HEK-293T cell lysate (AB259109)

  • 0
  • 0
Primary Antibodies

AB32454

Anti-MAP2 antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

  • 5
  • 35

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com