JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB67417

Anti-SH2D1B 抗体

Anti-SH2D1B antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Polyclonal SH2D1B antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SH2D1B.

別名を表示する

EAT2, SH2D1B, SH2 domain-containing protein 1B, EWS/FLI1-activated transcript 2, EAT-2

1 Images
Western blot - Anti-SH2D1B antibody (AB67417)
  • WB

Unknown

Western blot - Anti-SH2D1B antibody (AB67417)

All lanes:

Western blot - Anti-SH2D1B antibody (ab67417) at 1/500 dilution

Lane 1:

SH2D1B transfected 293T cell lysate at 25 µg/mL

Lane 2:

Non-transfected 293T cell lysate at 25 µg/mL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Key facts

宿主種

Mouse

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human SH2D1B.

O14796

特異性

This antibody is reactive against mammalian transfected lysate.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SH2D1B also known as EAT-2 is an adaptor protein that has a significant role in immune cell signaling. It is about 15 kDa in size and contains a single SH2 domain which allows it to interact with phosphorylated tyrosine-containing proteins. SH2D1B is mainly expressed in natural killer (NK) cells and some T-cell populations. In these cells SH2D1B modulates signaling pathways by interacting with various receptors and other signaling proteins affecting cellular responses.
Biological function summary

SH2D1B influences immune cell activation and effector functions. It is part of a signaling complex that modulates the activity of NK cells and some T-cells influencing processes like cytotoxicity and cytokine production. By participating in this complex SH2D1B plays a role in regulating immune responses affecting conditions ranging from viral infections to cancer surveillance where adaptive immunity is modified.

Pathways

SH2D1B is involved in the regulation of the SLAM family receptor signaling pathway which is vital for the activation and function of NK cells. It interacts closely with other proteins in this pathway such as SAP (SLAM-associated protein) to influence signaling cascades that determine cell fate and activity. SH2D1B also participates in the NF-kB signaling pathway further affecting immune responses through modulation of gene expression involved in cell survival and differentiation.

SH2D1B has associations with immune system dysfunctions such as X-linked lymphoproliferative syndrome (XLP). This disorder involves unregulated immune responses and is linked to an overactive or misregulated SLAM receptor signaling where SH2D1B collaborates with SAP. Additionally variations in SH2D1B expression or function have potential links to autoimmune diseases due to its role in regulatory pathways of immune activation and suppression.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as CD84, SLAMF1, LY9 and CD244 (PubMed : 11689425). In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation; downstream signaling involves PLCG1 and ERK activation (PubMed : 24687958). Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling (By similarity). In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse (PubMed : 24687958). Negatively regulates CD40-induced cytokine production in dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation (By similarity).
See full target information SH2D1B
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 15:735723 PubMed34658793

2021

The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Chen,Ruiying Qiang,Yuan Zhang,Wei Cao,Leilei Wu,Pei Jiang,Jingru Ai,Xiangyu Ma,Ying Dong,Xia Gao,He Li,Ling Lu,Shasha Zhang,Renjie Chai
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB222814

Anti-SH2D1B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D1B antibody (AB222814)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB77483

Anti-UXT antibody

Western blot - Anti-UXT antibody (AB77483)

  • 0
  • 0
Primary Antibodies

AB67886

Anti-ZNF331 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ZNF331 antibody (AB67886)

  • 0
  • 0
Primary Antibodies

AB153981

Anti-SGK3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SGK3 antibody (AB153981)

  • 0
  • 0
Primary Antibodies

AB140932

Anti-EDG8 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG8 antibody - N-terminal (AB140932)

  • 5
  • 3

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com