Anti-SFRP2 抗体 [EPR26088-157] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (AB318186)

This data was developed using ab318185, the same antibody clone in a different buffer formulation.

Low expression : spleen.

Lanes 1-3 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 4-5 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SFRP2 antibody [EPR26088-157] (<a href='/products/primary-antibodies/sfrp2-antibody-epr26088-157-ab318185'>ab318185</a>) at 1/1000 dilution

Lane 1:

Human eyeball tissue lysate at 30 µg

Lane 2:

Human breast tissue lysate at 30 µg

Lane 3:

Human spleen tissue lysate at 30 µg

Lane 4:

Untreated Y79 (human retinoblastoma cell) whole cell lysate at 30 µg

Lane 5:

Y79 treated with 300ng/ml Brefeldin A for 24 hours whole cell lysate at 30 µg

Secondary

Lanes 1 - 3:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 4 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa,36 kDa

false

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (AB318186)

This data was developed using ab318185, the same antibody clone in a different buffer formulation.

Dot blot analysis of SFRP2 using ab318185 at 1 : 1000 (0.501 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lane 1 : His-tagged human SFRP2 recombinant protein
Lane 2 : His-tagged human SFRP1 recombinant protein

Anti-6X His tag ® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 1000) as total protein control.

This antibody does not cross-react with human SFRP1.

All lanes:

Dot Blot - Anti-SFRP2 antibody [EPR26088-157] (<a href='/products/primary-antibodies/sfrp2-antibody-epr26088-157-ab318185'>ab318185</a>) at 1/1000 dilution

Lane 1:

His-tagged human SFRP2 recombinant protein

Lane 2:

His-tagged human SFRP1 recombinant protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s