AB233565

Anti-SF3B4 抗体 [3A1]

Name: Anti-SF3B4 抗体 [3A1] (ab233565) | Abcam
Brand: Abcam Limited
SKU: AB233565
Price: 70000 JPY
Availability: InStock

Anti-SF3B4 antibody [3A1]

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(1 Publication)

Mouse Monoclonal SF3B4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SF3B4.

別名を表示する

SAP49, SF3B4, Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3b 49 kDa subunit, Spliceosome-associated protein 49, SAP 49

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SF3B4 antibody [3A1] (AB233565)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SF3B4 antibody [3A1] (AB233565)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SF3B4 (red) using ab233565 at 1/1000 dilution in ICC/IF.

Chicken antibody to vimentin (green) and DNA (blue, stained with DAPI).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B4 antibody [3A1] (AB233565)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B4 antibody [3A1] (AB233565)

Formallin-fixed, paraffin-embedded human tonsil tissue stained for SF3B4 using ab233506 at 10 μg/ml in immunohistochemical analysis.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

3A1

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human SF3B4.

Q15427

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

Purified from TCS.

バッファー組成

Preservative: 0.07% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SF3B4 also known as SAP49 is a component of the spliceosome complex involved in pre-mRNA splicing. This protein weighs around 49 kDa and is expressed in various human tissues with especially high levels in the liver kidney and brain. SF3B4 functions by binding to pre-mRNA substrates and plays an essential role in recognizing splice sites an important step in the splicing process. SF3B4 along with other SF3B family members forms part of the U2 small nuclear ribonucleoprotein (snRNP) complex contributing to the accurate selection and removal of introns from pre-mRNA.

Biological function summary

SF3B4 participates in the precise editing and maturation of pre-mRNA during gene expression. As part of the U2 snRNP complex it collaborates with other factors in the assembly of the spliceosome. This complex ensures the precursor mRNA is properly spliced to produce mature mRNA closely linking SF3B4’s activity to efficient genetic information processing. SF3B4's function is especially important in ensuring the fidelity of the splicing mechanism which affects both mRNA stability and translational efficiency.

Pathways

The activity of SF3B4 is importantly tied to the RNA splicing and mRNA processing pathways. It interacts with other critical proteins like SF3B1 and SF3B2 which together facilitate the correct assembly and function of the spliceosome. This involvement in splicing pathways affects gene expression regulation an essential component of cellular differentiation and development. Disruption in SF3B4 or its associated proteins can lead to defective mRNA splicing impacting multiple downstream biological processes.

SF3B4’s function correlates with several splicing-related pathologies such as Nager syndrome and various cancers. Nager syndrome a rare congenital disorder involves mutations in SF3B4 that disrupt normal splicing processes. Additionally expression changes in SF3B4 have connections with oncogenesis and its interaction with other SF3B proteins contributes to aberrant splicing patterns often observed in cancerous cells. These interactions highlight its potential as a therapeutic target in disease treatment and management.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 10882114, PubMed : 12234937, PubMed : 27720643, PubMed : 32494006). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 12234937, PubMed : 32494006). Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed : 12234937, PubMed : 27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed : 12234937). May also be involved in the assembly of the 'E' complex (PubMed : 10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077, PubMed : 33509932).

See full target information SF3B4

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 84:104281 PubMed36162205

2022

Integrating microarray-based spatial transcriptomics and single-cell RNA-sequencing reveals tissue architecture in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Guo,Bolun Zhou,Zhenlin Yang,Xiang Liu,Qilin Huai,Lei Guo,Xuemin Xue,Fengwei Tan,Yin Li,Qi Xue,Shugeng Gao,Jie He

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SF3B4 抗体 [3A1]

Anti-SF3B4 antibody [3A1]

Immunocytochemistry/ Immunofluorescence - Anti-SF3B4 antibody [3A1] (AB233565)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B4 antibody [3A1] (AB233565)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise