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Anti-SF3B2 抗体 [5D2] (ab56800)

Reviews (1) Submit a question References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B2 antibody [5D2] (ab56800)
  • Western blot - Anti-SF3B2 antibody [5D2] (ab56800)
  • Immunocytochemistry/ Immunofluorescence - Anti-SF3B2 antibody [5D2] (ab56800)

Key features and details

  • Mouse monoclonal [5D2] to SF3B2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-SF3B2 antibody [5D2]

  • 製品の詳細

    Mouse monoclonal [5D2] to SF3B2

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment: ISLGMPVGPN AHKVPPPWLI AMQRYGPPPS YPNLKIPGLN SPIPESCSFG YHAGG, corresponding to amino acids 592-646 of Human SF3B2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.4
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    5D2

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab56800の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 98 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 98 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    SF3B2 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B2 may also be involved in the assembly of the 'E' complex. It also belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

  • 細胞内局在

    Nuclear

  • 参照データベース

    • 別名

      • Pre mRNA splicing factor SF3b 145 kDa subunit antibody
      • SAP 145 antibody
      • SAP145 antibody
      • SF3b1 antibody
      • SF3B145 antibody
      • SF3b150 antibody
      • Spliceosome associated protein 145 antibody
      • Splicing factor 3b subunit 2 145kDa antibody
      • Splicing factor 3b subunit 2 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B2 antibody [5D2] (ab56800)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B2 antibody [5D2] (ab56800)

      SF3B2 antibody (ab56800) used in immunohistochemistry at 6ug/ml on formalin fixed and paraffin embedded human kidney.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-SF3B2 antibody [5D2] (ab56800)
      Western blot - Anti-SF3B2 antibody [5D2] (ab56800)

      SF3B2 antibody (ab56800) at 1ug/lane + HeLa cell lysate at 25ug/lane.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-SF3B2 antibody [5D2] (ab56800)
      Immunocytochemistry/ Immunofluorescence - Anti-SF3B2 antibody [5D2] (ab56800)

      ab56800, at 60 µg/ml staining SF3B2 in HeLa Cells by immunocytochemistry. Immunofluorescence of ab56800 to SF3B2 on HeLa cell, performed with antibody concentration at 60 ug/ml.

      This image was generated using the ascites version of the product.

    参考文献 (3)

    ab56800 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab56800 は 3 報の論文で使用されています。

    • Simmler P  et al. SF3B1 facilitates HIF1-signaling and promotes malignancy in pancreatic cancer. Cell Rep 40:111266 (2022). PubMed: 36001976
    • Murthy T  et al. Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin. J Biol Chem 293:10220-10234 (2018). PubMed: 29764937
    • Lin-Moshier Y  et al. Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling. J Biol Chem 288:355-67 (2013). WB . PubMed: 23148228

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-SF3B2 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (BHK21-T7)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    80 µg
    Specification
    BHK21-T7
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Simon Boudreault

    Verified customer

    投稿 Sep 16 2020

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