Anti-SETD1B 抗体 [EPR25142-11] (BSA and Azide free) (ab300480)

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Western blot - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR25142-11] to SETD1B - BSA and Azide free
Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB
Reacts with: Mouse, Rat, Human

Unconjugated

製品名

Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free)
SETD1B 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR25142-11] to SETD1B - BSA and Azide free
由来種

Rabbit
アプリケーション

適用あり: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details
適用なし: IP
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: HeLa, HeLa transfected with siRNA specifically targeti SETD1B, whole, MCF7, F9, 293T, NIH/3T3 and PC-12 lysates. IHC-P: Human colon, Human colon cancer and Mouse liver cancer tissues. ICC: HeLa and F9 cells. Flow Cyt: HeLa and F9 cells.
特記事項
ab300480 is the carrier-free version of ab300479.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.2
Constituent: 100% PBS
キャリア・フリー

はい
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR25142-11
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-SETD1B antibody [EPR25142-11] (ab300479)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab300480の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		Use at an assay dependent concentration.
Flow Cyt (Intra)		1/50.
WB		Use at an assay dependent concentration.

特記事項
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt (Intra) 1/50.
WB Use at an assay dependent concentration.

追加情報

Is unsuitable for IP.

機能

Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.
配列類似性

Contains 1 post-SET domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SET domain.
細胞内局在

Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SET1B each bind to a unique set of target genes.
Target information above from: UniProt accession Q9UPS6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 23067 Human
- Entrez Gene: 208043 Mouse
- Omim: 611055 Human
- SwissProt: Q9UPS6 Human
- SwissProt: Q8CFT2 Mouse
- Unigene: 507122 Human
別名
- FLJ20803 antibody
- Histone-lysine N-methyltransferase SETD1B antibody
- hSET1B antibody
- KIAA1076 antibody
- KMT2G antibody
- Lysine N-methyltransferase 2G antibody
- SET domain containing 1B antibody
- SET domain-containing protein 1B antibody
- SET1B antibody
- SET1B_HUMAN antibody
- SETD1B antibody
see all

Western blot - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

All lanes : Anti-SETD1B antibody [EPR25142-11] (ab300479) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.

Exposure time: Lane1: 3 minutes, Lane 2: 26 seconds.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29138278).

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Western blot - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

All lanes : Anti-SETD1B antibody [EPR25142-11] (ab300479) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting SETD1B, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Exposure time: 3 minutes

This data was developed using ab300479, the same antibody clone in a different buffer formulation.

Exposure time: 3 minutes.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29138278).

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Western blot - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

All lanes : Anti-SETD1B antibody [EPR25142-11] (ab300479) at 1/1000 dilution

Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.

Exposure time: Lane 1 - 3 minutes, Lane 2: 26 seconds.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29138278).

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

An unknown band at ~115 kDa was detected in lane 3.
Immunocytochemistry/ Immunofluorescence - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (ab300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells lebelling SETD1B with ab300479 at 1/100 dilution (5.2 µg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in F9 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: PBS was used instead of primary antibody follwed by preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (ab300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells lebelling SETD1B with ab300479 at 1/100 dilution (5.2 µg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: PBS was used instead of primary antibody follwed by preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue labelling SETD1B with ab300479 at 1/100 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human colon at a low level (PMID: 24925220). The section was incubated with ab300479 at 4C overnight. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody followed by secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver cancer tissue labelling SETD1B with ab300479 at 1/100 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on mouse liver cancer. The section was incubated with ab300479 at 4C overnight. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody followed by secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labelling SETD1B with ab300479 at 1/100 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human colon cancer (PMID:24925220). The section was incubated with ab300479 at 4C overnight. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody followed by secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
Flow Cytometry (Intracellular) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.
Intracellular flowcytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling SETD1B with ab300479 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

This data was developed using ab300479, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling SETD1B with ab300479 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Anti-SETD1B antibody [EPR25142-11] (BSA and Azide free) (AB300480)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

ab300480 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300480 は論文での使用が確認できていません。

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

キャリア・フリー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

Certificate of Compliance

参考文献 (0)

レビューと Q&A