Anti-SETD1B 抗体 (ab113984)
Key features and details
- Rabbit polyclonal to SETD1B
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SETD1B antibody
SETD1B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SETD1B -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Cow, Chimpanzee, Gorilla
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免疫原
Synthetic peptide corresponding to Human SETD1B aa 350-400.
Database link: 23067 -
ポジティブ･コントロール
- HeLa cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab113984 is affinity purified using an epitope specific to SETD1B immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113984の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 10 µg/mg of lysate.
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| 特記事項 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 10 µg/mg of lysate. |
ターゲット情報
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機能
Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro. -
配列類似性
Contains 1 post-SET domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SET domain. -
細胞内局在
Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SET1B each bind to a unique set of target genes. - Information by UniProt
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参照データベース
- Entrez Gene: 23067 Human
- Omim: 611055 Human
- SwissProt: Q9UPS6 Human
- Unigene: 507122 Human
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別名
- FLJ20803 antibody
- Histone-lysine N-methyltransferase SETD1B antibody
- hSET1B antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD1B antibody (ab113984)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling SETD1B with ab113984 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-SETD1B antibody (ab113984)ab113984 used at 10 µg/mg of lysate staining SETD1B in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) by immunoprecipitation. For blotting immunoprecipitated SETD1B, ab113984 was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab113984 は 3 報の論文で使用されています。
- Lindner P et al. EMT transcription factor ZEB1 alters the epigenetic landscape of colorectal cancer cells. Cell Death Dis 11:147 (2020). PubMed: 32094334
- Yang P et al. SET domain containing 1B gene is mutated in primary hepatic neuroendocrine tumors. Int J Cancer 145:2986-2995 (2019). PubMed: 30977120
- Chen D et al. High-level SETD1B gene expression is associated with unfavorable prognosis in hepatocellular carcinoma. Mol Med Rep 19:1587-1594 (2019). PubMed: 30628696